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G006151

UHPLC/MS Analysis of Antineoplastic and Antidepressant Drugs in Plasma on Titan™ C18 after SPE using HybridSPE®-PLus

application for HPLC, application for SPE

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technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample/matrix: rat plasma
SPE well plate: HybridSPE-PLus 96-Well Plate, 50 mg/2 mL per well (575659-U)
sample preparation: final analyte concentration 50 ng/mL
sample addition: 400 μL of 1% formic acid acetonitile:spiked plasma (3:1) per well
column: Titan C18, 5 cm x 2.1 mm I.D., 1.9 μm particles (577122-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 90:10 (v:v) acetonitrile:water
gradient: 35 to 90% B in 1.5 min, held at 90% B for 0.4 min
flow rate: 0.4 mL/min
pressure: 3582 psi (247 bar)
column temp.: 35 °C
detector: TOF/MS
injection: 1 μL

suitability

application for HPLC
application for SPE

application(s)

pharmaceutical (small molecule)

Analysis Note

HybridSPE-Phospholipid plates removed plasma-derived proteins and phospholipids to maximize LC/MS sensitivity. Titan C18 U/HPLC columns provided rapid resolution. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany
Titan is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    Fluoxetine hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®
    Tamoxifen, ≥99%

SPE tube or plate

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standard

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