G006134
HPLC Analysis of Proteins on Proteomix® SCX Columns, Effect of Particle Size of Resolution
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)
column: Proteomix SCX-NP3, 5 cm x 4.6 mm I.D., 3 μm particles (Z777151)
mobile phase: (A) 20 mM sodium phosphate buffer, pH 6.0, (B) A + 1.0 M NaCl
gradient: 0-25 min from 0-75% B, 15 min prewash
flow rate: 0.75 mL/min
column temp.: Ambient
detector: 214 nm
injection: 5 μL
sample: each 1 mg/mL
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Legal Information
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analytical column
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