G006121
HPLC Analysis of Recombinant Tumor Necrosis Factor Receptor-Fc Fusion Protein (rhTNFR-Fc) on Zenix® SEC-300 versus SRT® SEC-300, Effect of Particle Size on Resolution
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm, 300 Å particles (Z777051)
column: SRT SEC-300, 30 cm x 7.8 mm I.D., 5 μm, 300 Å particles (Z777051) (Z777033)
mobile phase: 150 mM sodium phosphate, pH 7.0
flow rate: 1 mL/min
detector: UV, 214 nm
injection: 20 μL
sample: Recombinant tumor necrosis factor receptor-Fc fusion protein (rhTNFR-Fc), including oligomers (440 kD and 360 kD), monomer (150 kD), and impurity (120 kD)
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Legal Information
analytical column
standard
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