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Key Documents

G005854

LC/MS Analysis of Antiarrhythmic Drugs and Metabolites in Plasma on Ascentis® Express HILIC following Sample Prep using HybridSPE®-Phospholipid

application for HPLC

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24 EA
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24 EA
CN¥3,997.99

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technique(s)

HPLC: suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: rabbit plasma, unfiltered K2-EDTA, spiked with each compound at 100 ng/mL (3:1, plasma:1% formic acid in acetonitrile)
SPE tube/cartridge: HybridSPE-Phospholipid, 96-well plate (575656-U)
sample addition: to each well add 100 μL plasma, followed by a 300 μL of 1% formic acid in acetonitrile
elution: collect, filtrate and analyze directly.
column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile, 5:95 (A:B), adjusted to pH 7.0 with formic acid
flow rate: 0.4 mL/min
pressure: 1305 psi (90 bar)
column temp.: 35 °C
detector: ESI+, 100-1000 m/z
injection: 0.5 μL

suitability

application for HPLC

application(s)

clinical

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Corning® centrifuge bottle with standard screw cap, capacity 500 mL, polypropylene

CLS431845

Corning® centrifuge bottle

Corning® centrifuge bottle with standard screw cap, capacity 500 mL, polycarbonate

CLS431847

Corning® centrifuge bottle

Corning® centrifuge bottle with silicon O-ring screw cap, capacity 500 mL, polycarbonate

CLS431846

Corning® centrifuge bottle

material

flat bottom tube, round bottle, wide-mouth bottle, polypropylene , translucent bottle

material

flat bottom tube, polypropylene , round bottle, translucent bottle, wide-mouth bottle

material

clear bottle, flat bottom tube, polycarbonate , round bottle, wide-mouth bottle

material

clear bottle, flat bottom tube, polycarbonate , round bottle, wide-mouth bottle

manufacturer/tradename

Corning 431844

manufacturer/tradename

Corning 431845

manufacturer/tradename

Corning 431847

manufacturer/tradename

Corning 431846

packaging

pkg of 24 ea

packaging

pkg of 24 ea

packaging

pkg of 24 ea

packaging

pkg of 24 ea

capacity

500 mL

capacity

500 mL

capacity

500 mL

capacity

500 mL

feature

autoclavable

feature

autoclavable, cap

feature

autoclavable, cap

feature

autoclavable, cap

Analysis Note

This application shows the extraction and analysis of antiarrhythmic cardiac drugs in plasma samples using LC/MS/MS. HILIC chromatography on Ascentis Express along with a selective sample preparation using HybridSPE-Phospholipid provided sensitivity and resolution. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Lidocaine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
    Amiodarone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®
    N-Desethylamiodarone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®
    (±)-Flecainide solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

SPE tube or plate

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Description
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analytical column

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standard

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