Skip to Content
Merck
CN
All Photos(1)

Documents

G005611

HPLC Analysis of Withania Standards on Discovery® C18

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971)
mobile phase: [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient: 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate: 1.5 mL/min
pressure: 220 bar (3200 psi)
column temp.: 27 °C
detector: UV, 227 nm
injection: 20 μL
sample: 20 μg/mL in water: acetonitrile (86:14)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Discovery C18 for the efficient separation of withania standards by the USP monograph.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Withanoside IV
    Withanoside V
    Withaferin A
    12-Deoxywithastramonolide
    Withanolide A
    Withanone
    Withanolide B

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service