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G004641

HPLC Analysis of Luciferin Enantiomers on Astec® CHIROBIOTIC® V

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase: [A] 20 mM ammonium nitrate, pH 5.5; [B] THF; (90:10, A:B)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 254 nm
injection: 6 μL
sample: 0.5 mg/mL in methanol

suitability

application for HPLC

application(s)

chemicals and industrial polymers
life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

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