G004505
HPLC Analysis of Methylphenidate Enantiomers on Astec® CHIROBIOTIC® V
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase: [A] methanol; [B] 1% triethylamine, pH 4/1; (95:5, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 220 nm
injection: 2 μL
sample: 1 mg/mL in methanol
suitability
application for HPLC
application(s)
forensics and toxicology
pharmaceutical (small molecule)
Legal Information
Analyte
analytical column
standard
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