G001419
HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18
application for HPLC
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technique(s)
HPLC: suitable
test parameters
column: Discovery HS C18, 15 cm × 4.6 mm I.D., 3 μm particles (569252-U)
mobile phase: 100% acetonitrile
flow rate: 0.8 mL/min
column temp.: 30 °C
detector: UV, 290 nm
injection: 10 μL
sample: 50 μg/mL each analyte
suitability
application for HPLC
application(s)
clinical
vitamins, nutraceuticals, and natural products
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Analyte
Description
Ergocalciferol (D2), analytical standard
Cholecalciferol (D3), analytical standard
analytical column
Product No.
Description
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standard
Product No.
Description
Pricing
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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