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Discovery® C8 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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Synonym(s):
C8 Reversed-Phase Chromatography Column
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Discovery® C8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L7

product line

Discovery®

feature

endcapped

manufacturer/tradename

Discovery®

packaging

1 ea of

extent of labeling

7.5% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

15 cm × 4.6 mm

surface area

200 m2/g

surface coverage

3.4 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical base material
fully porous particle

matrix active group

C8 (octyl) phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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Application

Discovery® C8 HPLC column has been used in the separation of fluoroquinolone antibiotics in tablets using reversed-phase high-performance liquid chromatography (RP-HPLC).

Features and Benefits

  • Excellent reproducibility
  • Faster separation of strongly hydrophobic analytes than C18 columns
  • Stable, low-bleed LC-MS separations
  • Exceptional peak shapes for basic and acidic compounds
  • Compatible with low organic/highly aqueous mobile phases

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Regulatory Information

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rahul Palchaudhuri et al.
Cell reports, 13(9), 2027-2036 (2015-12-15)
Apoptosis is generally believed to be a process that requires several hours, in contrast to non-programmed forms of cell death that can occur in minutes. Our findings challenge the time-consuming nature of apoptosis as we describe the discovery and characterization
Laurence Conraux et al.
PloS one, 8(11), e79733-e79733 (2013-11-14)
The diagnostic of Amyotrophic lateral sclerosis (ALS) remains based on clinical and neurophysiological observations. The actual delay between the onset of the symptoms and diagnosis is about 1 year, preventing early inclusion of patients into clinical trials and early care
Eridan Orlando Pereira et al.
Biotechnology for biofuels, 6, 111-111 (2013-08-07)
Microorganisms are the most proficient decomposers in nature, using secreted enzymes in the hydrolysis of lignocellulose. As such, they present the most abundant source for discovery of new enzymes. Acremonium alcalophilum is the only known cellulolytic fungus that thrives in
Jun-Yan Zhang et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 37(21), 3268-3270 (2013-02-13)
To establish a method for determing the content of two isomers containd in Garcinia hanburyi by HPLC. Chromatographic column of SunFire (Waters) C8 (2.1 mm x 150 mm, 3.5 microm) was adopted, with acetonitrile-methanol-0.3% trifluoroacetic acid (36: 37:27) as the
T Mazzei et al.
The Journal of antimicrobial chemotherapy, 31 Suppl C, 1-9 (1993-03-01)
After the discovery of erythromycin and other natural compounds, including oleandomycin, spiramycin, josamycin and midecamycin, much research has been devoted to synthesizing derivatives or analogues with improved chemical, biological and pharmacokinetic properties. These new macrolides are semisynthetic molecules that differ

Protocols

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

Related Content

Discovery C18 and C8 HPLC Columns products offered

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