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Supelco

SUPELCOSIL LC-18-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 10 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

SUPELCOSIL LC-18-DB HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm

Agency

suitable for USP L1

feature

endcapped

manufacturer/tradename

SUPELCOSIL

extent of labeling

11.0% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 10 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide shorter retention, better peak shape, and higher efficiency for organic bases than can be obtained on other Type A silica reversed-phase columns.

Application

SUPELCOSIL LC-18-DB HPLC Column has been used for the separation of:
  • Analytes of pharmaceutical relevance during their analysis using LC in conjunction with principal component analysis (PCA).
  • Uracil in aqueous solutions during its separation by reversed phase high performance liquid chromatography equipped with ultraviolet-visible (UV-Vis) detection.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Regulatory Information

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Characterisation of reversed-phase liquid chromatographic columns by chromatographic tests. Evaluation of 36 test parameters: repeatability, reproducibility and correlation.
Visky D, et al.
Journal of Chromatography A, 977(1), 39-58 (2002)
Ce Li et al.
American journal of physiology. Gastrointestinal and liver physiology, 320(3), G380-G395 (2021-01-28)
Pancreatic ductal adenocarcinoma (PDAC) is highly metastatic and represents one of the deadliest forms of human cancers. Previous studies showed that activation of Yes-associated protein 1 (YAP1) plays a key role in malignant transformation in the pancreas. In this study
Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns using principal component analysis
Euerby MR and Petersson P
Journal of Chromatography A, 994(1-2), 13-36 (2003)
E Terjéki et al.
Journal of pharmaceutical and biomedical analysis, 24(5-6), 913-920 (2001-03-15)
RGH-1756 (1-(2-methoxy-phenyl)-4-(4-[4-(6-imidazo[2,1-b]-thiazolyl)-phenoxy]-butyl)-piperazine dimethansulphonate) is a novel atypical antipsychotic candidate of Gedeon Richter Ltd. A new HPLC method has been developed and validated for the quantitative determination of RGH-1756 in dog and rat plasma. The compound and the internal standard are
J S Patrick et al.
Analytical biochemistry, 199(1), 125-131 (1991-11-15)
A rapid, isocratic method for the determination of tryptophan in Escherichia coli fermentation broths by reversed-phase HPLC is described. Tryptophan can be measured in fermentations containing either chemically defined media or media with hydrolyzed protein supplements. The procedure was rugged

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