Anti-p-PDHA1-Ser232 Antibody, clone 2D5 ZooMAb^® Rabbit Monoclonal

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ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 2D5 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Pyruvate dehydrogenase E1 (PDHA1) phosphorylated on serine 232.

KLH-conjugated linear peptide corresponding to 10 amino acids surrounding phosphoserine 232 from human Pyruvate dehydrogenase E1 (PDHA1).

Quality Control Testing

Evaluated by Western Blotting in Rat liver tissue lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected PDHA1 phosphorylated on Ser 232 in Rat liver tissue lysate.

Tested applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected p-PDHA1-Ser232 in Human and Mouse liver tissue sections.

Peptide Inhibition Assay Analysis: Target band detection in Rat liver tissue lysate was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p-PDHA1-Ser232 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Rat liver tissue lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected PDHA1 phosphorylated on Ser 232 in Rat liver tissue lysate.

Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (UniProt: P08559; also known as EC:1.2.4.1, PDHE1-A type I) is encoded by the PDHA1 (also known as PHE1A) gene (Gene ID: 5160) in human. The pyruvate dehydrogenase complex (PDC) is a tightly organized arrangement of polypeptides and cofactors that work in a sequential manner in the multi-enzyme complex catalyzing a multi-step reaction in which pyruvate is converted into acetyl-CoA and CO2. The intermediate substrates and products remain bound to the complex, increasing overall efficiency. This complex contains three catalytic enzymes (E1, E2, E3), two regulatory enzymes, and a binding protein and requires cofactors TPP, lipoic acid, and FAD. E1 (PDHA1) is a ubiquitously distributed mitochondrial matrix protein that is synthesized with a mitochondrial transit peptide (aa 1-29), which is subsequently cleaved off to produce the mature active form. It is considered as a rate limiting step of PDC. Its activity is regulated by phosphatases that activate it and kinases that inhibit the enzyme activity. Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family of kinases is known to inactivate the enzyme and phosphorylation at a single site is sufficient to cause this inactivation. However, dephosphorylation at all three sites is required for reactivation. E2 is responsible for transferring the hydroxyethyl group from TPP to an oxidized form of covalently bound lipoamide, and the resulting acetyl group is then transferred to free coenzyme A to form acetyl-CoA and reduced dihydrolipoamide-E2. E3 re-oxidizes the lipoyl group of dihydrolipoamide-E2 to form lipoamide-E2 and NADH. Mutations in PDHA1 gene are known to cause E1 deficiency that results in primary lactic acidosis in children. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kato, M., et al. (2008). Structure. 16(12); 1849-1859; Korotchkina, LG., and Patel, MS. (2001). J. Biol. Chem. 276(40); 37223-37229).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.