XNAPE
REDExtract-N-Amp™ Plant PCR Kit
sufficient for 100 extractions, sufficient for 500 amplifications
Synonym(s):
Plant direct PCR kit, Plant direct PCR kit with loading dye
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About This Item
UNSPSC Code:
41106303
NACRES:
NA.55
usage
sufficient for 100 extractions
sufficient for 500 amplifications
sufficient for 500 reactions
Quality Level
feature
dNTPs included
hotstart
color
red
shipped in
wet ice
storage temp.
−20°C
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General description
The REDExtract-N-Amp™ Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix™ contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Application
REDExtract-N-Amp™ Plant PCR Kit has been used to extract genomic DNA from plants and to perform polymerase chain reaction.
Features and Benefits
- Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
- No freezing, mechanical disruption, organic extraction, column purification or precipitation required
- Specially formulated PCR ReadyMix for use with extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysis
- Compatible with high-throughput requirements for genetic analysis of plants
- Extract stable at 4 °C for at least 6 months
Components
Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Regulatory Information
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Ralf G Kynast et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(26), 9921-9926 (2004-06-16)
We have developed from crosses of oat (Avena sativa L.) and maize (Zea mays L.) 50 fertile lines that are disomic additions of individual maize chromosomes 1-9 and chromosome 10 as a short-arm telosome. The whole chromosome 10 addition is
Mark A Jones et al.
Journal of experimental botany, 58(6), 1261-1270 (2007-02-16)
Reactive oxygen species (ROS) production by an NADPH oxidase (NOX) encoded by AtrbohC/RHD2 is required for root hair growth in Arabidopsis thaliana. ROP (RHO of plants) GTPases are also required for normal root hair growth and have been proposed to
Yuri Jorge Peña Ramírez et al.
Clinical and vaccine immunology : CVI, 14(6), 685-692 (2007-04-27)
The human immunodeficiency virus type 1 (HIV-1) Tat protein is considered a potential candidate vaccine antigen. In an effort to design a strategy for noninvasive vaccination against HIV-1, we developed transgenic tomatoes expressing the Tat protein. Two independent plants testing
Anna Stöger et al.
Journal of microbiological methods, 58(2), 281-284 (2004-07-06)
Angular leaf spot is a bacterial disease caused by Xanthomonas fragariae. It has become a serious disease in the USA and Europe in recent years. Several detection procedures are described for this plant pathogen. However, they are either too time-consuming
Avinash C Srivastava et al.
Plant physiology, 148(1), 200-211 (2008-07-25)
AtSUC2 (At1g22710) encodes a phloem-localized sucrose (Suc)/H(+) symporter necessary for efficient Suc transport from source tissues to sink tissues in Arabidopsis (Arabidopsis thaliana). AtSUC2 is highly expressed in the collection phloem of mature leaves, and its function in phloem loading
Articles
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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