XNAP
REDExtract-N-Amp™ Plant PCR Kit
sufficient for 100 extractions, sufficient for 100 amplifications
Sign Into View Organizational & Contract Pricing
All Photos(3)
Synonym(s):
Plant direct PCR kit, Plant direct PCR kit with loading dye
UNSPSC Code:
12352200
NACRES:
NA.55
Recommended Products
usage
sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions
Quality Level
feature
dNTPs included
hotstart
technique(s)
PCR: suitable
color
red
shipped in
wet ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
General description
The REDExtract-N-Amp™ Plant PCR Kits enable the rapid detection of plant pathogens by direct PCR (polymerase chain reaction). These kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by direct PCR. The REDExtract-N-Amp PCR ReadyMix™ contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.
Application
REDExtract-N-Amp™ Plant PCR Kit has been used to:
- extract genomic DNA from mycelial lawns and leaves
- screen leaf tissue from putative transgenic potato plants
- perform polymerase chain reaction (PCR)
- isolate DNA from Cannabis sativa L. seed and dried fruit body gills
Features and Benefits
- Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
- No freezing, mechanical disruption, organic extraction, column purification or precipitation required
- Specially formulated PCR ReadyMix for use with extract
- Hot Start antibody for highly specific PCR amplification of genomic DNA
- REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysis
- Compatible with high-throughput requirements for genetic analysis of plants
- Extract stable at 4 °C for at least 6 months
Components
Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Rebeca Cobos et al.
Applied and environmental microbiology, 81(18), 6474-6483 (2015-07-15)
Grapevine trunk fungal pathogens, such as Diplodia seriata and Phaeomoniella chlamydospora, can infect plants through pruning wounds. They cause grapevine trunk diseases and are involved in grapevine decline. Accordingly, the protection of pruning wounds is crucial for the management of
Yolanda Arana-Gabriel et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(3), 632-640 (2018-02-28)
The present study conducted a genetic characterization and determined growth rate and biomass production in solid and liquid media, using strains obtained from wild edible sporomes of Lyophyllum that grow in high mountains. Vegetative isolation was used to obtain a
Samuel J Harrison et al.
Plant methods, 2, 19-19 (2006-11-08)
The floral dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium is the method of choice for Arabidopsis transformation. The presence of a marker, usually antibiotic- or herbicide-resistance, allows identification of transformed seedlings from untransformed seedlings.
Giuseppe Forlani et al.
Forensic science international. Genetics, 52, 102464-102464 (2021-01-19)
The availability of molecular markers able to distinguish drug-type from fiber-type Cannabis sativa cultivars would allow fast and cheap analysis of any plant specimen, including seeds and leaves. Several approaches to this issue have been described, mainly using polymorphisms in
Siobhan M Brady et al.
Molecular systems biology, 7, 459-459 (2011-01-20)
Tightly controlled gene expression is a hallmark of multicellular development and is accomplished by transcription factors (TFs) and microRNAs (miRNAs). Although many studies have focused on identifying downstream targets of these molecules, less is known about the factors that regulate
Articles
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Related Content
Explore technology and reagent portfolios for plant breeding workflows, accelerating the development of new crop varieties.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service