TGO
ThermaGo™
increases PCR specificity, inhibits non-specific polymerase activity, prevents mis-priming during PCR
Synonym(s):
Multiplex PCR, PCR Additive, hot start DNA polymerase, hot start PCR, hotstart
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About This Item
form
lyophilized powder
packaging
units of 2500 units, units of 500 units, pkg of One unit of ThermaGo™ is defined as the amount required for maximum improvements in amplification reactions containing 1 unit of Taq DNA polymerase in a volume of 25 μl.
technique(s)
PCR: suitable
color
opaque
shipped in
ambient
storage temp.
2-8°C
General description
Application
- monoplex and multiplexed end-point single nucleotide polymorphism (SNP) genotyping
- detection of low-copy target numbers during single-cell PCR and digital PCR
- detection of low-copy target numbers in liquid biopsies/tumor samples
- enhancing the accuracy of multiplex PCR for next-generation sequencing and other post PCR applications
Features and Benefits
- Acts at higher temperatures during annealing and elongation steps
- Improves the reproducibility
- Detects low-copy number targets by suppressing non-specific targets
- Minimizes signal scatter in digital & standard PCR
- Minimizes mispriming and chimera production before next-generation sequencing (NSG)
- Enhances end-point genotyping
Preparation Note
Legal Information
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Articles
How to stop nonspecific bands and off target primers in PCR.
PCR additive added to master mix that stops nonspecific bands after cool down.
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
ThermaStop™ 和 ThermaGo™ PCR 添加剂可抑制扩增前、扩增中和扩增后的错配，从而提高PCR的特异性和灵敏度。ThermaStop™-RT添加剂在一步法和两步法RT-PCR中可作为逆转录酶的热启动剂。
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