T8944
Topoisomerase II α human
(Single band on SDS-PAGE), liquid
Synonym(s):
Topoisomerase II alpha, TOPO® II, p170
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Quality Level
form
liquid
mol wt
170 kDa
UniProt accession no.
storage temp.
−70°C
Gene Information
human ... TOP2A(7153)
General description
Topoisomerase II α (TopoIIα) is a gene product with conserved catalytic activities and it promotes the progression of DNA damage. The α isoform is present in proliferating cells.
Topoisomerase II α is encoded by TOP2A gene, which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication, recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer, ovarian cancer and prostate cancer.
Application
Human topoisomerase II α has been used in DNA cleavage assay.
Topoisomerase II α has been used in a study to assess aging processes in the human brain. Topoisomerase II α has also been used in a study to investigate its activity in HIV-1 replication.
Biochem/physiol Actions
Used to study DNA structure and topology.
Unit Definition
One unit will decatenate 0.2 μg of kinetoplast DNA in 30 minutes at 37°C.
Physical form
Buffered aqueous glycerol solution, containing Trizma buffer (pH 7.7). Other components are present in the solution, and lot-specific information on these components may be obtained from Technical Service.
Preparation Note
Recombinant enzyme overexpressed in a proprietary eukaryotic cell line.
Legal Information
TOPO is a registered trademark of Life Technologies
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
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J R Spitzner et al.
Nucleic acids research, 16(12), 5533-5556 (1988-06-24)
Topoisomerase II, purified from chicken erythrocytes, was reacted with a large number of different DNA fragments and cleavages were catalogued in the presence and absence of drugs that stabilize the cleavage intermediate. Cleavages were sequenced to derive a consensus for
TOP2A induces malignant character of pancreatic cancer through activating beta-catenin signaling pathway.
Pei YF, et al.
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(1), 197-207 (2018)
Maryjean Pendleton et al.
Annals of the New York Academy of Sciences, 1310, 98-110 (2014-02-06)
Type II topoisomerases are essential enzymes that modulate DNA under- and overwinding, knotting, and tangling. Beyond their critical physiological functions, these enzymes are the targets for some of the most widely prescribed anticancer drugs (topoisomerase II poisons) in clinical use.
M Vanni et al.
Poultry science, 93(4), 856-863 (2014-04-08)
Escherichia coli are a common inhabitant of the gastrointestinal tract of mammals and birds; nevertheless, they may be associated with a variety of severe and invasive infections. Whereas fluoroquinolones (FQ) have been banned in the United States for use in
The prognostic significance of topoisomerase II alpha protein in early stage luminal breast cancer.
An X, et al.
BMC Cancer, 18(1), 331-331 (2018)
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