Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(1)

Key Documents

View All Documentation

Safety Information

T8665

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Synonym(s):

TMB

Sign Into View Organizational & Contract Pricing

Select a Size

1 G
CN¥4,564.92

CN¥4,564.92

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(1)

Select a Size

Change View
1 G
CN¥4,564.92

About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

CN¥4,564.92

Please contact Customer Service for Availability
Request a Bulk Order

Product Name

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System, peroxidase substrate

Quality Level

300

form

liquid

storage condition

protect from light

shipped in

wet ice

storage temp.

2-8°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
54790U5477554784
LiChrospher® Si-60 HPLC Column 5 μm particle size, L × I.D. 25 cm × 4.6 mm

54792

LiChrospher® Si-60 HPLC Column

LiChrospher® Si-60 HPLC Column 5 μm particle size, L × I.D. 15 cm × 4.6 mm

54790U

LiChrospher® Si-60 HPLC Column

LiChrospher® RP-18 HPLC Column 5 μm particle size, L × I.D. 15 cm × 3.2 mm

54775

LiChrospher® RP-18 HPLC Column

Supelco

54784

LiChrospher® NH2 HPLC Column

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

reversed phase

separation technique

anion exchange, hydrophilic interaction (HILIC), normal phase

matrix active group

silica phase

matrix active group

silica phase

matrix active group

C18 (octadecyl) phase

matrix active group

amino phase

matrix

silica gel

matrix

silica, fully porous particle

matrix

octadecyl, silica particle platform

matrix

amino

pore size

60 Å

pore size

60 Å

pore size

100 Å

pore size

100 Å

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

Application

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.
3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Caution

This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.

Physical form

Ready-to-use.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 3 - STOT SE 1

Target Organs

Eyes,Central nervous system

WGK

WGK 2

Regulatory Information

危险化学品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000429455
    0000429455
    0000252796
    0000252796
    SLCP9930

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Janine Wesslowski et al.
    The Journal of biological chemistry, 295(26), 8759-8774 (2020-05-10)
    The Wingless/Int1 (Wnt) signaling system plays multiple, essential roles in embryonic development, tissue homeostasis, and human diseases. Although many of the underlying signaling mechanisms are becoming clearer, the binding mode, kinetics, and selectivity of 19 mammalian WNTs to their receptors
    Sui-Yuan Chang et al.
    Scientific reports, 11(1), 8692-8692 (2021-04-24)
    A metal nanoparticle composite, namely TPNT1, which contains Au-NP (1 ppm), Ag-NP (5 ppm), ZnO-NP (60 ppm) and ClO2 (42.5 ppm) in aqueous solution was prepared and characterized by spectroscopy, transmission electron microscopy, dynamic light scattering analysis and potentiometric titration. Based on the in
    S Madersbacher et al.
    Methods (San Diego, Calif.), 21(1), 41-50 (2000-04-15)
    As a glycoprotein hormone, human chorionic gonadotropin (hCG) is not a single molecular entity. This term comprises not only the bioactive heterodimer hCG but also an array of molecular protein backbone and glycosylation variants, such as its free beta (hCGbeta)
    Tomasz Brudek et al.
    Molecular neurodegeneration, 12(1), 44-44 (2017-06-09)
    Parkinson's' disease (PD) and Multiple System Atrophy (MSA) are progressive brain disorders characterized by intracellular accumulations of α-synuclein and nerve cell loss in specific brain areas. This loss causes problems with movement, balance and/or autonomic functions. Naturally occurring autoantibodies (NAbs)
    Paweł Kozielewicz et al.
    Molecular pharmacology, 97(1), 23-34 (2019-11-11)
    Smoothened (SMO) is a GPCR that mediates hedgehog signaling. Hedgehog binds the transmembrane protein Patched, which in turn regulates SMO activation. Overactive SMO signaling is oncogenic and is therefore a clinically established drug target. Here we establish a nanoluciferase bioluminescence
    View All Related Papers

    Articles

    Colorimetric Phosphatase/Peroxidase Substrate Systems

    NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service