T7924
Transforming Growth Factor-α human
TGF-α, recombinant, expressed in E. coli, powder, suitable for cell culture
Synonym(s):
hTGF-α, TGF-α
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About This Item
Empirical Formula (Hill Notation):
C239H348N70O72S6
CAS Number:
Molecular Weight:
5546.14
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥98% (SDS-PAGE and HPLC)
form
powder
potency
≤0.2 ng/mL ED50
quality
endotoxin tested
mol wt
5.5 kDa
packaging
pkg of 1 mg
pkg of 5 mg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... TGFA(7039)
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Amino Acid Sequence
Val-Val-Ser-His-Phe-Asn-Asp-Cys-Pro-Asp-Ser-His-Thr-Gln-Phe-Cys-Phe-His-Gly-Thr-Cys-Arg-Phe-Leu-Val-Gln-Glu-Asp-Lys-Pro-Ala-Cys-Val-Cys-His-Ser-Gly-Tyr-Val-Gly-Ala-Arg-Cys-Glu-His-Ala-Asp-Leu-Leu-Ala [Disulfide bridges: 8-21; 16-32; 34-43]
General description
The TNFα (tumor necrosis factor α) gene is mapped to human chromosome 6p21.33. TNFα is a member of TNF superfamily.
Application
Transforming Growth Factor-α human has been used:
- to determine the ideal concentration of growth factors to be used in the culture medium that supports sheep preantral follicles
- in human hepatocytes cell culture and keratinocyte cell culture
- to induce morphogenesis in primary mouse organoids
Biochem/physiol Actions
The TNFA gene is significantly associated with the regulation of innate and adaptive immune responses. Polymorphism of TNFA gene is known to cause acute motor axonal neuropathy. The cytokine release and gene action is associated with epithelial cells, myeloid cells, endothelial, as well as tumor cells. Tumor cells are known to release TNFA, specifically in response to chemotherapy.
Transforming growth factor-α (TGF-α), is a 5.5 kDa polypeptide containing 50 amino acids in its mature form. It was originally identified as an agent that reversibly confers a transformed phenotype upon normal non-neoplastic cells, such as normal rat kidney fibroblasts. This activity requires the presence of transforming growth factor-β (TGF-β), which potentiates the action of TGF-α via a separate receptor. TGF-α is synthesized by monocytes, keratinocytes and many tissues and tumors. Mice genetically engineered to be lacking TGF-α expression showed no abnormalities except for the detection of a "wavy" hair coat. TGF-α exerts its action through the EGF receptor.
Physical form
Lyophilized from a 0.2 μm filtered buffered solution.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorpation into BALB/c 3T3 cells.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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