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T7451

Sigma-Aldrich

Anti-Acetylated Tubulin antibody, Mouse monoclonal

clone 6-11B-1, purified from hybridoma cell culture

Synonym(s):

Acetylated Tubulin Antibody, Acetylated Tubulin Antibody - Monoclonal Anti-Acetylated Tubulin antibody produced in mouse, Anti Acetyl Tubulin antibody, Anti Acetyl-alpha tubulin

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Select a Size

25 μL
CN¥2,257.75
100 μL
CN¥5,169.41
200 μL
CN¥8,231.20

CN¥2,257.75

Available to ship onApril 27, 2025Details

Request a Bulk Order
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25 μL
CN¥2,257.75
100 μL
CN¥5,169.41
200 μL
CN¥8,231.20

About This Item

MDL number:
MFCD00164512
UNSPSC Code:
12352203
NACRES:
NA.41

CN¥2,257.75

Available to ship onApril 27, 2025Details

Request a Bulk Order

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6-11B-1, monoclonal

form

buffered aqueous solution

species reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

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This Item
T5168T6793T6074
Anti-Acetylated Tubulin antibody, Mouse monoclonal clone 6-11B-1, purified from hybridoma cell culture

T7451

Anti-Acetylated Tubulin antibody, Mouse monoclonal

Monoclonal Anti-α-Tubulin antibody produced in mouse clone B-5-1-2, ascites fluid

T5168

Monoclonal Anti-α-Tubulin antibody produced in mouse

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse clone 6-11B-1, ascites fluid

T6793

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse

Anti-α-Tubulin antibody, Mouse monoclonal clone B-5-1-2, purified from hybridoma cell culture

T6074

Anti-α-Tubulin antibody, Mouse monoclonal

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

bovine, frog, invertebrates, human, hamster, mouse, protista, pig, monkey, chicken, rat, plant

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

species reactivity

mouse, protista, invertebrates, hamster, plant, frog, rat, monkey, pig, bovine, chicken, human

species reactivity

mouse, chicken, Chlamydomonas, African green monkey, human, rat, bovine, sea urchin, kangaroo rat

clone

6-11B-1, monoclonal

clone

B-5-1-2, monoclonal

clone

6-11B-1, monoclonal

clone

B-5-1-2, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α -tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.
Monoclonal anti-acetylated tubulin antibody (mouse IgG2b isotype) is derived from the hybridoma 6-11B-1 produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin). Tubulin is a heterodimer, which consists of a-tubulin and b-tubulin; both subunits have a molecular weight of 50,000 and share considerable homology.

Specificity

The antibody has been used to detect acetylated α-tubulins from many organisms including protista, plants, invertebrates, and vertebrates. The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corre­sponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila, and yeast. Occasionally, the epitope recognized by the antibody may be absent or masked, as it is in the rat kangaroo epithelial-like cell line PtK2.

Immunogen

acetylated tubulin from the outer arm of Strongylocentrotus purpuratus (sea urchin).

Application

Monoclonal Anti-Acetylated Tubulin antibody produced in mouse has been used in:
  • quantitative dot blot
  • immunofluorescence
  • Western blot
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • solid-phase radioimmunoassay (RIA)
  • electron microscopy
Anti-acetylated tubulin antibody has also been used in staining X-gal–stained paraffin sections to perform immunohistochemistry.[1]

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

常规特殊物品
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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000398828
    0000398829
    0000346262
    0000352392
    0000312701

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    Heike Olbrich et al.
    American journal of human genetics, 97(4), 546-554 (2015-09-22)
    Multiciliated epithelial cells protect the upper and lower airways from chronic bacterial infections by moving mucus and debris outward. Congenital disorders of ciliary beating, referred to as primary ciliary dyskinesia (PCD), are characterized by deficient mucociliary clearance and severe, recurrent
    Inhibition of histone deacetylases 1 and 6 enhances cytarabine-induced apoptosis in pediatric acute myeloid leukemia cells.
    Xu X
    PLoS ONE, 6(2) (2011)
    Heterozygous loss-of-function SEC61A1 mutations cause autosomal-dominant tubulo-interstitial and glomerulocystic kidney disease with anemia
    Bolar NA, et al.
    American Journal of Human Genetics, 99(1), 174-187 (2016)
    Basal cells as stem cells of the mouse trachea and human airway epithelium.
    Rock JR
    Proceedings of the National Academy of Sciences of the USA, 106(31), 12771-12775 (2009)
    G Piperno et al.
    The Journal of cell biology, 104(2), 289-302 (1987-02-01)
    The subcellular distribution of microtubules containing acetylated alpha-tubulin in mammalian cells in culture was analyzed with 6-11B-1, a monoclonal antibody specific for acetylated alpha-tubulin. Cultures of 3T3, HeLa, and PtK2 cells were grown on coverslips and observed by immunofluorescence microscopy
    View All Related Papers

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