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General description
The Synaptic Vesicles Isolation Kit provides all the reagents required for preparing an enriched synaptic vesicle fraction. The isolation procedure involves tissue homogenization, hypo-osmotic lysis of the synaptosomes, and differential centrifugation followed by an OptiPrep density gradient. The kit also contains an antibody specific for synaptophysin, a synaptic vesicle marker, to follow the enrichment procedure. The kit was tested on rat and rabbit brain tissues.
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