SRP6053
ProBDNF human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
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Synonym(s):
Abrineurin, BDNF, Brain-derived neurotrophic factor
UNSPSC Code:
12352202
NACRES:
NA.32
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General description
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors. The precursor form of brain-derived neurotrophic factor (pro-BDNF) undergoes post-translational cleavage to form mature BDNF (mBDNF). The gene encoding this protein is localized on human chromosome 11p14.1.
Biochem/physiol Actions
Precursor form of brain-derived neurotrophic factor (pro-BDNF) interacts preferentially with the pan-neurotrophin receptor p75 (p75NTR) (2) and vps10p domain-containing receptor sortilin and induces neuronal apoptosis, whereas mature BDNF selectively binds with high affinity to the tropomyosin-related kinase B (TrkB) kinase receptor and promotes the survival, growth and differentiation of neurons. As proneurotrophins and mature neurotrophins elicit opposite biological effects, pro-BDNF cleavage in the neuronal system is regulated in a specific and cell-context dependent manner. Pro-BDNF plays important role in negative regulation of neurotrophic actions in the brain.
Physical form
Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.
Preparation Note
Centrifuge the vial prior to opening.
Reconstitution
Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.
Other Notes
Recombinant Human precursor form of Brain-derived neurotrophic factor produced in E. coli is a non-glycosylated polypeptide chain containing 2X229 amino acids as a homodimer and having a molecular mass of 52 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Met-Lys-Glu.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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The presence of a single-nucleotide polymorphism in the BDNF gene affects the rate of locomotor adaptation after stroke.
Helm EE
Experimental Brain Research. Experimentelle Hirnforschung. Experimentation Cerebrale, 234(2), 341-351 (2016)
Function and evolution in the NGF family and its receptors.
Ebendal T
Journal of Neuroscience Research, 32(4), 461-470 (1992)
11p14.1 microdeletions associated with ADHD, autism, developmental delay, and obesity.
Shinawi M
American Journal of Medical Genetics. Part A, 155A(6), 1272-1280 (2011)
Hao-Yun Zhang et al.
Biochimica et biophysica acta, 1843(12), 2967-2975 (2014-09-23)
Iron accumulation is observed in the substantia nigra of patients with Parkinson's disease. However, it is unknown whether neurotrophic factors, brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) participate in the modulation of neuronal iron metabolism. Here
Guoqi Zhu et al.
British journal of pharmacology, 172(9), 2354-2368 (2015-01-07)
Mild cognitive deficit in early Parkinson's disease (PD) has been widely studied. Here we have examined the effects of memantine in preventing memory deficit in experimental PD models and elucidated some of the underlying mechanisms. I.p. injection of 1-methyl-4- phenyl-1,2,3,6-tetrahydro
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