SRP5172
Catenin β, GST tagged human
recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
CTNNB, FLJ25606
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About This Item
biological source
human
recombinant
expressed in baculovirus infected Sf9 cells
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~115 kDa
technique(s)
activity assay: suitable
solubility
water: soluble
suitability
suitable for molecular biology
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CTNNB1(1499)
General description
Research area: Apoptosis. β-catenins are cytoplasmic proteins that are ubiquitously expressed. These proteins are associated with E-cadherin at cellular junctions. CTNNB1 (catenin β-1) belongs to the armadillo family of proteins. This protein possesses a 130 amino acid N-terminal domain, 12 imperfect repeats of 42 amino acids termed arm repeats, and a 100 amino acid C-terminal domain. The gene encoding β-catenin is localized on human chromosome 3p22.1.
Application
Catenin β, GST tagged human has been used:
- as a component of protein kinase buffer for in vitro kinase assay
- in in vitro citrullination assay to characterize β-catenin citrullination
- to perform differentiation factor screening
Biochem/physiol Actions
β-catenin interacts with T-cell factor and lymphoid enhancer-binding factor (TCF and LEF) transcription factors and is an essential member of the Wingless-Wnt signal transduction pathway. The adenomatous polyposis coli (APC) tumor-suppressor protein, together with Axin and glycogen synthase kinase 3 β (GSK3β), form a Wnt-regulated signaling complex that mediates phosphorylation-dependent degradation of βcatenin by the proteasome. APC and the Siah-1 E3 ubiquitin-protein ligase-1 mediate a novel β-catenin degradation pathway linking p53 activation to cell cycle control. Activating mutations in the human β-catenin gene have been found in human colon cancer and melanomas. The protein has a role in homeostasis and development.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
? catenin in health: A review
Sharada Prakash
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons (2015)
??-Catenin pathway activation in breast cancer
is associated with triple-negative phenotype
but not with CTNNB1 mutation
is associated with triple-negative phenotype
but not with CTNNB1 mutation
Felipe
Modern Pathology (2010)
Regulation of ?-Catenin Function by the I?B Kinases*
Carmela Lamberti
The Journal of Biological Chemistry (2001)
C Arbore et al.
Nature communications, 13(1), 1146-1146 (2022-03-05)
α-catenin is a crucial protein at cell junctions that provides connection between the actin cytoskeleton and the cell membrane. At adherens junctions (AJs), α-catenin forms heterodimers with β-catenin that are believed to resist force on F-actin. Outside AJs, α-catenin forms
Somatic mutations of the ?-catenin gene are frequent in mouse and human hepatocellular carcinomas
Alix de La Coste
Proceedings of the National Academy of Sciences of the USA (1998)
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