SRP5012
CDK9/CyclinK, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
C-2k, CCNK, CDC2L4, CPR4, MGC9113, PITALRE, TAK
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
15-21 nmol/min·mg
mol wt
~67 kDa (CyclinK)
~68 kDa (CDK9)
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CCNK(8812), CDK9(1025)
General description
CDK9/CyclinK, active, GST tagged human comprises recombinant full-length human CDK9 and cyclinK proteins co-expressedin Sf9 insect cells with an N-terminal GST tag. Cyclin-dependent protein kinase 9 (CDK9)/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is a component of the multiprotein complex mitogen-activated protein kinase 7 and transcription elongation factor β (TAK/P-TEFβ).
Biochem/physiol Actions
CDKs are closely related to yeast CDk1, cdc28 and cdc2 and arean important regulators of the cell cycle. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the human immune deficiency virus 1 (HIV-1) transactivating regulatory protein (Tat) protein, which suggests a possible involvement of this protein in AIDS.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Ranadip Mandal et al.
Cellular and molecular life sciences : CMLS, 79(12), 597-597 (2022-11-19)
Cervical cancer is the fourth most frequently diagnosed and fatal gynecological cancer. 15-61% of all cases metastasize and develop chemoresistance, reducing the 5-year survival of cervical cancer patients to as low as 17%. Therefore, unraveling the mechanisms contributing to metastasis
Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation
Kim YK, et al.
Molecular and Cellular Biology, 22(13), 4622-4637 (2002)
The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription
Yang Z, et al.
Nature, 414(6861), 317-322 (2001)
F Bullrich et al.
Cancer research, 55(6), 1199-1205 (1995-03-15)
Orderly progression through the cell cycle requires sequential activation and inactivation of cyclin-dependent kinases (cdks). This is achieved in part through the association of cdks with positive regulators called cyclins and inactivation of cyclin-cdk complexes by a rapidly growing number
Z Yang et al.
Nature, 414(6861), 317-322 (2001-11-20)
The human positive transcription elongation factor P-TEFb, consisting of a CDK9/cyclin T1 heterodimer, functions as both a general and an HIV-1 Tat-specific transcription factor. P-TEFb activates transcription by phosphorylating RNA polymerase (Pol) II, leading to the formation of processive elongation
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