SRP3255
TPO from rat
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
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Synonym(s):
MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand
UNSPSC Code:
12352202
NACRES:
NA.75
Recommended Products
biological source
rat
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.2 ng/mL ED50
mol wt
18.7 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
rat ... THPO(81811)
Related Categories
General description
Thrombopoietin (TPO) also known as c-Mpl ligand is an acidic glycoprotein expressed mainly in the liver, kidney, and bone marrow. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kDa), which contains the erythropoietin-like domain of the full length TPO protein.
Application
Thrombopoietin (TPO) from rat has been used in experimental systems to determine the effect of TPO in recovery from thrombocytopenia.
Biochem/physiol Actions
Thrombopoietin (TPO) acts as a key physiologic regulator of megakaryocyte and platelet production in vivo. Mutation of TPO or its receptor might lead to thrombocytopenia and megakaryocytopoiesis. In addition, TPO also influence the action of erythroid and erythromegakaryocytic progenitors in mouse embryonic yolk sac. TPO binds to c-mpl receptor and regulates circulating platelet level.
Sequence
SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF
Physical form
Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Reactivation of a hematopoietic endocrine program of pregnancy contributes to recovery from thrombocytopenia.
Bhattacharyya S
Molecular Endocrinology, 16(6), 1386-1393 (2002)
Native thrombopoietin: structure and function.
Kato T
Stem Cells, 16(5), 322-328 (1998)
Recombinant human thrombopoietin: basic biology and evaluation of clinical studies.
Kuter DJ and Begley CG
Blood, 100(10), 3457-3469 (2002)
Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.
Foster DC
Proceedings of the National Academy of Sciences of the USA, 91(26), 13023-13027 (1994)
Thrombopoietin: the primary regulator of platelet production.
Kaushansky K
Blood, 86(2), 419-431 (1995)
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