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SRP3255

TPO from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

MGDF, Megakaryocyte colony-stimulating factor, Thrombopoietin, c-MPL Ligand

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About This Item

NACRES:
NA.75
UNSPSC Code:
12352202

Key Documents

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Product Name

TPO from rat, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

biological source

rat

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.2 ng/mL ED50

mol wt

18.7 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

P49745

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... THPO(81811)

Related Categories

Application

Thrombopoietin (TPO) from rat has been used in experimental systems to determine the effect of TPO in recovery from thrombocytopenia.

Biochem/physiol Actions

Thrombopoietin (TPO) acts as a key physiologic regulator of megakaryocyte and platelet production in vivo. Mutation of TPO or its receptor might lead to thrombocytopenia and megakaryocytopoiesis. In addition, TPO also influence the action of erythroid and erythromegakaryocytic progenitors in mouse embryonic yolk sac. TPO binds to c-mpl receptor and regulates circulating platelet level.

General description

Thrombopoietin (TPO) also known as c-Mpl ligand is an acidic glycoprotein expressed mainly in the liver, kidney, and bone marrow. Recombinant rat TPO is a fully biologically active 174 amino acid polypeptide (18.7kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Other Notes

SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
0209466

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Reactivation of a hematopoietic endocrine program of pregnancy contributes to recovery from thrombocytopenia.
Bhattacharyya S
Molecular Endocrinology, 16(6), 1386-1393 (2002)
Cloning and expression of murine thrombopoietin cDNA and stimulation of platelet production in vivo.
Lok S
Nature, 369(6481), 565-568 (1994)
Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization.
Foster DC
Proceedings of the National Academy of Sciences of the USA, 91(26), 13023-13027 (1994)
Native thrombopoietin: structure and function.
Kato T
Stem Cells, 16(5), 322-328 (1998)
Recombinant human thrombopoietin: basic biology and evaluation of clinical studies.
Kuter DJ and Begley CG
Blood, 100(10), 3457-3469 (2002)
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