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Key Documents

Safety Information

SRP3182

Sigma-Aldrich

VEGF human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen, VPF, Vascular Endothelial Growth Factor-A

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
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biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-8.0 ng/mL

mol wt

38.2 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

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This Item
SRP3183SRP3185SRP3184
VEGF human Animal-component free, recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

SRP3182

VEGF human

VEGF-B human recombinant, expressed in E. coli, &#8805;98% (SDS-PAGE), &#8805;98% (HPLC), suitable for cell culture

SRP3183

VEGF-B human

VEGF-D human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE), &#8805;95% (HPLC), suitable for cell culture

SRP3185

VEGF-D human

VEGF-C human recombinant, expressed in HEK 293 cells, &#8805;95% (SDS-PAGE), &#8805;95% (HPLC), suitable for cell culture

SRP3184

VEGF-C human

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

assay

≥95% (HPLC), ≥95% (SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

VEGF (vascular endothelial growth factor) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.[1] Recombinant human VEGF is a 38.2kDa disulfide-linked homodimeric protein consisting of two 165 amino acid polypeptide chains.

Application

VEGF human has been used as a chemoattractant for HUVECs (human umbilical vein endothelial cells) migration assay.[2]

Biochem/physiol Actions

VEGF (vascular endothelial growth factor) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis.[3] Substantial evidence implicates VEGF in the induction of tumor metastasis and intraocular neovascular syndromes.[4][5] Polymorphism in VEGF at C(-634)G is linked with diabetic retinopathy in type 2 diabetes.[6]

Sequence

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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    VB24041108
    111810044
    1118
    0518AFC10
    0116AFC10

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    M Clauss et al.
    The Journal of experimental medicine, 172(6), 1535-1545 (1990-12-01)
    Systemic infusion of low concentrations of tumor necrosis factor/cachectin (TNF) into mice that bear TNF-sensitive tumors leads to activation of coagulation, fibrin formation, and occlusive thrombosis exclusively within the tumor vascular bed. To identify mechanisms underlying the localization of this
    Vegf, Vegf-B, Vegf-C and their receptors KDR, FLT-1 and FLT-4 during the neoplastic progression of human colonic mucosa.
    Andre T
    International Journal of Cancer. Journal International Du Cancer, 86, 174-181 (2000)
    Naoko Takubo et al.
    Scientific reports, 9(1), 9304-9304 (2019-06-28)
    Vascular endothelial cells (ECs) in angiogenesis exhibit inhomogeneous collective migration called "cell mixing", in which cells change their relative positions by overtaking each other. However, how such complex EC dynamics lead to the formation of highly ordered branching structures remains
    Naoki Horikawa et al.
    Clinical cancer research : an official journal of the American Association for Cancer Research, 23(2), 587-599 (2016-07-13)
    High VEGF expression in ovarian cancer is an unfavorable prognostic factor. However, the role of VEGF in tumor immunity remains unclear. Here, we examined the impact of VEGF on local immunity, including induction of myeloid-derived suppressor cells (MDSC), in ovarian
    Mohammad Abdul-Ghani et al.
    Cell research, 27(10), 1195-1215 (2017-08-09)
    The post-natal heart adapts to stress and overload through hypertrophic growth, a process that may be pathologic or beneficial (physiologic hypertrophy). Physiologic hypertrophy improves cardiac performance in both healthy and diseased individuals, yet the mechanisms that propagate this favorable adaptation
    View All Related Papers

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