SRP0253
4EBP1 Active human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE)
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Synonym(s):
EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1
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biological source
human
recombinant
expressed in E. coli
Assay
≥70% (SDS-PAGE)
form
aqueous solution
mol wt
13.6 kDa
packaging
pkg of 100 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... EIF4EBP1(1978)
General description
The gene E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is mapped to human chromosome 8p11-p12. It belongs to the translation repressor protein family.
Human E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1)(GenBank Accession No. NM_004095), full length with N-terminal His tag, MW = 13.6kDa, expressed in Escherichia coli.
Human E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1)(GenBank Accession No. NM_004095), full length with N-terminal His tag, MW = 13.6kDa, expressed in Escherichia coli.
Application
4EBP1 Active human has been used as a substrate for in vitro kinase assay.
Biochem/physiol Actions
E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is involved in protein synthesis, cell growth and survival. In unphosphorylated form, it suppresses the translation initiation of capped mRNAs. On the other hand, in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eIF4E (eukaryotic translation initiation factor 4E), thereby resulting in cap-dependent translation. E1F4EBP1 is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, it interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21.
Physical form
Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 50% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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Hiroshi Nishida et al.
Nucleic acids research, 37(17), 5678-5689 (2009-07-25)
ATM and ATR protein kinases play a crucial role in cellular DNA damage responses. The inhibition of ATM and ATR can lead to the abolition of the function of cell cycle checkpoints. In this regard, it is expected that checkpoint
Chao Zhang et al.
PloS one, 4(3), e4881-e4881 (2009-03-19)
Deregulation of the phosphatidylinositol 3-kinases (PI3K)/Akt/mammalian target of rapamycin (mTOR) pathway plays a central role in tumor formation and progression, providing validated targets for cancer therapy. S9, a hybrid of alpha-methylene-gamma-lactone and 2-phenyl indole compound, possessed potent activity against this
Naoto Tatewaki et al.
PloS one, 11(1), e0147570-e0147570 (2016-01-30)
Ataxia telangiectasia mutated (ATM) kinase plays a crucial role as a master controller in the cellular DNA damage response. Inhibition of ATM leads to inhibition of the checkpoint signaling pathway. Hence, addition of checkpoint inhibitors to anticancer therapies may be
MicroRNAs 125a and 125b inhibit ovarian cancer cells through post-transcriptional inactivation of EIF4EBP1.
Lee M, et al.
Oncotarget, 7, 8726-8726 (2016)
Multiple interacting oncogenes on the 8p11-p12 amplicon in human breast cancer.
Yang ZQ, et al.
Cancer Research, 66, 11632-11632 (2006)
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