SRP0235
PI3 kinase [p110a(H1047R)/p85a] Active human
recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonym(s):
PIK3CA, Phosphoinositide-3-kinase, catalytic α, PtdIns-3-kinase p110
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
biological source
human
recombinant
expressed in baculovirus infected insect cells
Assay
≥60% (SDS-PAGE)
form
aqueous solution
specific activity
≥1770 pmol/min-μg
mol wt
129 kDa (p110α)
88 kDa (p85α)
packaging
pkg of 20 μg
concentration
0.38 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... PIK3CA(5290)
General description
Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Unit Definition
One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
WGK
WGK 2
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
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