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SRP0234

Sigma-Aldrich

PI3 kinase [p110a(E545K)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥55% (SDS-PAGE)

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Synonym(s):
PIK3CA, Phosphoinositide-3-kinase, catalytic, alpha, PtdIns-3-kinase p110
UNSPSC Code:
12352200
NACRES:
NA.77

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥55% (SDS-PAGE)

form

aqueous solution

specific activity

≥800 pmol/min-μg

mol wt

129 kDa
 88 kDa

packaging

pkg of 20 μg

concentration

0.56 mg/mL

NCBI accession no.

U79143 (p110alpha)
XM_043865 (p85alpha)

UniProt accession no.

P42336

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PIK3CA(5290)

General description

Complex of N-terminal FLAG-tagged recombinant full-length human p110 (GenBank Accession No. U79143), with E545K mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

WGK

WGK 1

Regulatory Information

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