Monoclonal Anti-MHC Class II-FITC antibody produced in rat

Major histocompatibility complex II (MHCII) is specifically localized on antigen presenting cells, such as macrophages, dendritic cells and B cells. MHC class II are classified in to two types classical and non- classical MHC class II molecules. In humans, there are three classical class II molecules HLA-DP, -DQ and –DR and two non-classical molecules, namely HLA-DM and –DO.

The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Activated C57BL/6 mouse spleen cells

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 4 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Major histocompatibility complex II (MHCII) plays a vital role in initiating immune response by presenting peptides derived from extracellular pathogens to T cells bearing the CD4 marker. Mutations in the gene increase the risk of susceptibility to autoimmune diseases such as diabetes mellitus, rheumatoid arthritis and pemphigus vulgaris.

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.