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Safety Information

SAB4504517

Sigma-Aldrich

Anti-phospho-PKR (pThr446) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-DYT33, Anti-EIF2AK1, Anti-LEUDEN, Anti-PKR, Anti-PPP1R83, Anti-PRKR

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 62 kDa

species reactivity

rat, human, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
western blot: 1:500-1:1000

NCBI accession no.

P19525

UniProt accession no.

P19525

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pThr446)

Gene Information

human ... EIF2AK2(5610)

General description

EIF2AK2 (eukaryotic translation initiation factor 2α kinase 2) is an intracellular protein, that is also called as RNA-dependent protein kinase (PKR). It belongs to the eIF2αfamily of protein kinases. PKR contains two RNA binding domains and a kinase domain. This gene is located on human chromosome 2p22-p21.

Immunogen

The antiserum was produced against synthesized peptide derived from human PKR around the phosphorylation site of Thr446.

Immunogen Range: 413-462

Biochem/physiol Actions

EIF2AK2 (eukaryotic translation initiation factor 2 α kinase 2) plays a key role in antiviral response promoted by interferons. This gene is involved in stress responses and inflammation pathways. This gene controls the development and multiplication of cells, nutrient signaling and metabolism. EIF2AK2 also participates in several cell regulatory pathways like antiviral activity, growth suppression, transcriptional regulation and apoptosis.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulation of PKR by RNA: formation of active and inactive dimers
Husain B, et al.
Biochemistry, 54(44), 6663-6672 (2015)
Polymorphism in the NLRP3 inflammasome-associated EIF2AK2 gene and inflammatory bowel disease
Varghese GP, et al.
Molecular Medicine Reports, 11(6), 4579-4584 (2015)
Yimeng Gao et al.
Immunity, 52(6), 1007-1021 (2020-06-05)
N6-methyladenosine (m6A) is the most abundant RNA modification, but little is known about its role in mammalian hematopoietic development. Here, we show that conditional deletion of the m6A writer METTL3 in murine fetal liver resulted in hematopoietic failure and perinatal

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