Skip to Content
Merck
CN
All Photos(10)

Documents

Safety Information

SAB4504264

Sigma-Aldrich

Anti-phospho-RAD51 (pTyr315) antibody produced in rabbit

affinity isolated antibody

Sign Into View Organizational & Contract Pricing
All Photos(10)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 36 kDa

species reactivity

rat, human, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
western blot: 1:500-1:1000

NCBI accession no.

Q06609

UniProt accession no.

Q06609

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr315)

Gene Information

human ... RAD51(5888)

General description

RAD51 (RAD51 recombinase) belongs to the RecA/RadA/Rad51 protein family. It has a conserved ATP binding core. This protein is expressed in testis, ovary, and lymphoid tissue. It is located on human chromosome 15q15.

Immunogen

The antiserum was produced against synthesized peptide derived from human RAD51 around the phosphorylation site of Tyr315.

Immunogen Range: 281-330

Application

Anti-phospho-RAD51 (pTyr315) antibody has been used in western blotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

RAD51 (RAD51 recombinase) plays a major role in homology-directed repair (HDR) and cell viability.This gene acts an an important target for cancer therapy. RAD51 interacts with BRCA2 (breast cancer susceptibility protein) and participates in DSB (double strand DNA breaks) repair.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Alteration/deficiency in activation-3 (Ada3) plays a critical role in maintaining genomic stability
Mirza S, et al.
Cell Cycle, 11(22), 4266-4274 (2012)
Detection of loss of heterozygosity at RAD51, RAD52, RAD54 and BRCA1 and BRCA2 loci in breast cancer: pathological correlations.
Gonzalez R, et al.
British Journal of Cancer, 81(3), 503-509 (1999)
The Rad51/RadA N-terminal domain activates nucleoprotein filament ATPase activity.
Galkin VE, et al.
Structure, 14(6), 983-992 (2006)
Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro.
Baumann P, et al.
Cell, 87(4), 757-766 (1996)
RAD51, BRCA2 and DNA repair: a partial resolution.
Lord CJ and Ashworth A
Nature Structural and Molecular Biology, 14(6), 461-462 (2007)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service