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SAB4300253

Anti-phospho-SMAD3 (pSer425) antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Anti-DKFZp586N0721 antibody produced in rabbit, Anti-DKFZp686J10186 antibody produced in rabbit, Anti-HSPC193 antibody produced in rabbit, Anti-HsT17436 antibody produced in rabbit, Anti-SMAD family member 3 antibody produced in rabbit

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
Species reactivity:
mouse, human, rat
Citations:
27
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
Uniprot accession no.:
P84022

Key Documents

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Product Name

Anti-phospho-SMAD3 (pSer425) antibody produced in rabbit, affinity isolated antibody

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~52 kDa

species reactivity

mouse, human, rat

concentration

1 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100

isotype

IgG

immunogen sequence

(C-S-S-V-SP)

NCBI accession no.

NP_001138574.1

UniProt accession no.

P84022

application(s)

research pathology

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer425)

Quality Level

100

Gene Information

human ... SMAD3(4088)

Related Categories

Application

Anti-phospho-SMAD3 (pSer425) antibody produced in rabbit has been used in immunoblotting.

Biochem/physiol Actions

Smad3 (mothers against decapentaplegic homolog 3) is associated with TGF-β (transforming growth factor β) signaling pathway, a cellular process regulating the homeostasis, development, and repair of cartilage. It is known to induce extracellular matrix production. Smad proteins are responsible for the transduction of signals from cell surface to the nucleus. Phosphorylation of Smad3 controls tumor progression, inflammation, fibrosis, obesity and diabetes. Smad3 expression might be associated with the pathogenesis of osteoarthritis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

General description

Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
The SMAD3 (mothers against decapentaplegic homolog 3) gene is mapped to human chromosome 15q22.33. Smad3 protein identifies tandem repeats of the palindromic sequence GTCTAGAC, which is part of TGF-β (Transforming growth factor β) signaling promoter region.

Immunogen

Peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S(p)), according to the protein SMAD3.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
951411325

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Down-regulation of microRNA-216b inhibits IL-1β-induced chondrocyte injury by up-regulation of Smad3
He J, et al.
Bioscience Reports, 37(2) (2017)
Histone deacetylase 3 unconventional splicing mediates endothelial-to-mesenchymal transition through transforming growth factor ?2
Zeng L, et al.
The Journal of Biological Chemistry, 288(44), 31853-31866 (2013)
Tianpeng Xu et al.
Cytotechnology, 71(1), 57-65 (2019-01-02)
Mesenchymal stem cells (MSCs) hold great potential to treat tissue damage based on their multipotent property, and are also considered as suitable cell resources to create tissue-engineered grafts for tendon repair. However, the clinical application of MSCs is still limited
Smad3 and Bmal1 regulate p21 and S100A4 expression in myocardial stromal fibroblasts via TNF-α.
Sato F, et al.
Histochemistry and Cell Biology, 148(6), 617-624 (2017)
Signaling via Smad2 and Smad3 is dispensable for adult murine hematopoietic stem cell function in vivo
Billing M, et al.
Experimental Hematology, 55, 34-44 (2017)
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