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SAB4200720

Sigma-Aldrich

Anti-β-Catenin antibody, Mouse monoclonal

clone 15B8, purified from hybridoma cell culture

Synonym(s):

Anti-CTNNB1, Anti-armadillo

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

15B8, monoclonal

form

buffered aqueous solution

mol wt

antigen ~94 kDa

species reactivity

chicken, mouse, human, rat, monkey, hamster, bovine, canine

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using Madin-Darby bovine kidney (MDBK) cell line extract
immunofluorescence: 5-10 μg/mL using human breast adenocarcinoma MCF7 cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

UniProt accession no.

P35222

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CTNNB1(1499)
mouse ... Ctnnb1(12387)
rat ... Ctnnb1(84353)

General description

Anti-β-Catenin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the 15B8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant chicken β-Catenin. β-Catenin, also known as Catenin βfamily.

Immunogen

recombinant chicken ß-Catenin

Application

Anti-β-Catenin antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry
  • immunoprecipitation

Biochem/physiol Actions

β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway. In adherens junctions, β-Catenin associates with the cytoplasmic domain of the cadherin family adhesion receptors, anchoring them to the actin cytoskeleton and thus acting as structural protein. β-Catenin interacts with T cell factor (TCF)- family transcription factors to activate target genes. Wnt/β-catenin signaling interacts with several cellular pathways in context-dependent ways, including JakSTAT (Janus kinase−Signal transducer and activator of transcription) cytokine, TGFβ (transforming growth factor−β), DNA damage and the Hippo signaling pathways. Furthermore, β-Catenin is involved in embryonic morphogenesis and tumorigenesis and is tightly regulated by phosphorylation and further degradation by the ubiquitin-proteasome system.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Thomas Andl et al.
Development (Cambridge, England), 131(10), 2257-2268 (2004-04-23)
Bone morphogenetic protein (BMP) signaling is thought to perform multiple functions in the regulation of skin appendage morphogenesis and the postnatal growth of hair follicles. However, definitive genetic evidence for these roles has been lacking. Here, we show that Cre-mediated
Kiyomi Tsuji-Tamura et al.
FEBS letters, 596(13), 1686-1699 (2022-04-02)
Blood vessels normally remain stable over the long term. However, in atherosclerosis, vascular cells leave the quiescent state and enter an activated state. Here, we investigated the factors that trigger the breakage of the quiescent state by screening growth factors
E Sadot et al.
Oncogene, 19(16), 1992-2001 (2000-05-10)
Beta-catenin and plakoglobin are closely related armadillo family proteins with shared and distinct properties; Both are associated with cadherins in actin-containing adherens junctions. Plakoglobin is also found in desmosomes where it anchors intermediate filaments to the desmosomal plaques. Beta-catenin, on
Beyond canonical: the Wnt and beta-catenin story
Moon RT and Gough NR
Science Signaling, 9(422), eg5-eg5 (2016)

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