SAB4200502
Anti-NONO antibody, Mouse monoclonal
clone NC5, purified from hybridoma cell culture
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All Photos(2)
Synonym(s):
Monoclonal Anti-52 kDa subunit, Monoclonal Anti-54 kDa nuclear RNA- and DNA-binding protein, Monoclonal Anti-55 kDa nuclear protein, Monoclonal Anti-DNA-binding p52/p100 complex, Monoclonal Anti-NMT55, Monoclonal Anti-NONO antibody produced in mouse, Monoclonal Anti-NRB54, Monoclonal Anti-P54, Monoclonal Anti-P54NRB, Monoclonal Anti-non-POU domain-containing octamer (ATGCAAAT) binding protein, Monoclonal Anti-p54(nrb)
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NC5, monoclonal
form
buffered aqueous solution
species reactivity
mouse, rat
concentration
~1.0 mg/mL
technique(s)
indirect immunofluorescence: 1-2 μg/mL using whole extracts of mouse Hepa1-6 cells.
western blot: 0.5-1.0 μg/mL using whole extracts of mouse Hepa1-6 cells.
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
mouse ... Nono(53610)
rat ... Nono(317259)
General description
Monoclonal Anti-NONO (mouse IgG2a isotype) is derived from the hybridoma NC5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Non-POU-domain-containing, octamer binding protein (NONO), also known as p54nrb, is a member of the DBHS (Drosophila behavior, human splicing) protein family. The family also consists of paraspeckle protein 1 (PSPC1) and splicing factor proline- and glutamine-rich (SFPQ). NONO, PSPC1 and SFPQ are the core protein components of paraspeckles.
Specificity
Monoclonal Anti-NONO recognizes mouse NONO.
Immunogen
Synthetic peptide corresponding to a sequence at the C-terminal region of mouse NONO, conjugated to KLH.1 The corresponding sequence is identical in rat and differs by a single amino acids in human.
Application
Monoclonal Anti-NONO antibody produced in mouse has been used in immunohistochemistry and immunoblotting.
Biochem/physiol Actions
Non-POU-domain-containing, octamer binding protein (NONO) complexes with androgen receptor and activates androgen receptor-mediated transcription. NONO is necessary for cAMP-dependent activation of cAMP response element-binding protein (CREB) target genes. It acts as a bridge between the CREB/ transducers of regulated CREB (TORC) complex and RNA polymerase II. NONO associates with Period circadian protein homolog 1 (PERIOD-1) protein and is essential for the maintenance of circadian rhythm. NONO along with the nuclear factor, polypyrimidine tract-binding protein-associated splicing factor (PSF) binds to defective RNA and are capable of their retention.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Yu-Ting Huang et al.
PloS one, 12(11), e0187276-e0187276 (2017-11-02)
Synchronized bursts (SBs) with complex structures are common in neuronal cultures. Although the phenomenon of SBs has been discovered for a long time, its origin is still unclear. Here, we investigate the properties of these SBs in cultures grown on
Seungwoo Kang et al.
Neuropharmacology, 129, 47-56 (2017-11-13)
Alcoholism and psychiatric disorders like depression and anxiety are often comorbid. Although the mechanisms underlying this comorbidity are unclear, emerging evidence suggests that maladaptation of the glial glutamate transporter GLT-1 may play a role. Findings from animal and human studies
Neat1 in hematopoietic stem cells
Fallik N, et al.
Oncotarget, 8(65), 109575-109575 (2017)
Kazuhisa Akiba et al.
Molecular reproduction and development, 87(11), 1124-1125 (2020-10-07)
This study provides supporting evidence for the association between SOX9 and liquid-liquid phase separation. We show that SOX9 colocalized with a paraspeckle protein NONO in many, but not all, of the immortalized and primary murine Sertoli cells examined. In addition
Lei-lei Chen et al.
Acta pharmacologica Sinica, 33(3), 385-392 (2012-01-24)
To investigate the effects of rapamycin on glutamate uptake in cultured rat astrocytes expressing N-terminal 552 residues of mutant huntingtin (Htt-552). Primary astrocyte cultures were prepared from the cortex of postnatal rat pups. An astrocytes model of Huntington's disease was
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