Skip to Content
Merck
CN
All Photos(2)

Key Documents

View All Documentation

Safety Information

SAB4200502

Sigma-Aldrich

Anti-NONO antibody, Mouse monoclonal

clone NC5, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-52 kDa subunit, Monoclonal Anti-54 kDa nuclear RNA- and DNA-binding protein, Monoclonal Anti-55 kDa nuclear protein, Monoclonal Anti-DNA-binding p52/p100 complex, Monoclonal Anti-NMT55, Monoclonal Anti-NONO antibody produced in mouse, Monoclonal Anti-NRB54, Monoclonal Anti-P54, Monoclonal Anti-P54NRB, Monoclonal Anti-non-POU domain-containing octamer (ATGCAAAT) binding protein, Monoclonal Anti-p54(nrb)

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

NC5, monoclonal

form

buffered aqueous solution

species reactivity

mouse, rat

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: 1-2 μg/mL using whole extracts of mouse Hepa1-6 cells.
western blot: 0.5-1.0 μg/mL using whole extracts of mouse Hepa1-6 cells.

isotype

IgG2a

UniProt accession no.

Q99K48

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

mouse ... Nono(53610)
rat ... Nono(317259)

General description

Monoclonal Anti-NONO (mouse IgG2a isotype) is derived from the hybridoma NC5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Non-POU-domain-containing, octamer binding protein (NONO), also known as p54nrb, is a member of the DBHS (Drosophila behavior, human splicing) protein family. The family also consists of paraspeckle protein 1 (PSPC1) and splicing factor proline- and glutamine-rich (SFPQ). NONO, PSPC1 and SFPQ are the core protein components of paraspeckles.

Specificity

Monoclonal Anti-NONO recognizes mouse NONO.

Immunogen

Synthetic peptide corresponding to a sequence at the C-terminal region of mouse NONO, conjugated to KLH.1 The corresponding sequence is identical in rat and differs by a single amino acids in human.

Application

Monoclonal Anti-NONO antibody produced in mouse has been used in immunohistochemistry and immunoblotting.

Biochem/physiol Actions

Non-POU-domain-containing, octamer binding protein (NONO) complexes with androgen receptor and activates androgen receptor-mediated transcription. NONO is necessary for cAMP-dependent activation of cAMP response element-binding protein (CREB) target genes. It acts as a bridge between the CREB/ transducers of regulated CREB (TORC) complex and RNA polymerase II. NONO associates with Period circadian protein homolog 1 (PERIOD-1) protein and is essential for the maintenance of circadian rhythm. NONO along with the nuclear factor, polypyrimidine tract-binding protein-associated splicing factor (PSF) binds to defective RNA and are capable of their retention.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
112M4779V

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Neat1 in hematopoietic stem cells
Fallik N, et al.
Oncotarget, 8(65), 109575-109575 (2017)
Kazuhisa Akiba et al.
Molecular reproduction and development, 87(11), 1124-1125 (2020-10-07)
This study provides supporting evidence for the association between SOX9 and liquid-liquid phase separation. We show that SOX9 colocalized with a paraspeckle protein NONO in many, but not all, of the immortalized and primary murine Sertoli cells examined. In addition
Yu-Ting Huang et al.
PloS one, 12(11), e0187276-e0187276 (2017-11-02)
Synchronized bursts (SBs) with complex structures are common in neuronal cultures. Although the phenomenon of SBs has been discovered for a long time, its origin is still unclear. Here, we investigate the properties of these SBs in cultures grown on
Seungwoo Kang et al.
Neuropharmacology, 129, 47-56 (2017-11-13)
Alcoholism and psychiatric disorders like depression and anxiety are often comorbid. Although the mechanisms underlying this comorbidity are unclear, emerging evidence suggests that maladaptation of the glial glutamate transporter GLT-1 may play a role. Findings from animal and human studies
Lei-lei Chen et al.
Acta pharmacologica Sinica, 33(3), 385-392 (2012-01-24)
To investigate the effects of rapamycin on glutamate uptake in cultured rat astrocytes expressing N-terminal 552 residues of mutant huntingtin (Htt-552). Primary astrocyte cultures were prepared from the cortex of postnatal rat pups. An astrocytes model of Huntington's disease was
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service