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SAB4200477

Sigma-Aldrich

Anti-ALIX (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-PDCD6IP, programmed cell death 6 interacting protein, AIP1, DRIP4, HP95, ALG-2 interacting protein 1, Anti-apoptosis-linked gene 2-interacting protein X, Anti-dopamine receptor interacting protein 4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~95 kDa

species reactivity

human, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 1-2 μg using lysates of human Jurkat cells
indirect immunofluorescence: 2.5-5 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of mouse A20 cells

UniProt accession no.

Q8WUM4

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PDCD6IP(10015)
mouse ... Pdcd6ip(18571)

General description

The gene PDCD6IP (programmed cell death 6 interacting protein), also known as ALIX (apoptosis-linked gene 2-interacting protein X), is mapped to human chromosome 3p22.1 The protein has a crescent-shaped Bro1 domain, V-shaped V domain and a proline-rich region.

Specificity

Anti-ALIX (C-terminal) recognizes human and mouse ALIX.

Immunogen

peptide corresponding to a sequence at the C-terminal region of human ALIX/ PDCD6IP, conjugated to KLH. The corresponding sequence is identical in mouse, pig, monkey, bovine and dog and differs by a single amino acid in rat.

Application

Anti-ALIX (C-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Apoptosis-linked gene 2-interacting protein X (ALIX) protein binds to endophilin proteins that regulate membrane shape during endocytosis. Over-expression of the ALIX gene and endophilin leads to cytoplasmic vacuolization, which may be a reason for protection against cell death.
PDCD6IP (programmed cell death 6 interacting protein) interacts with PDCD6, a protein involved in apoptosis. It also participates in intraluminal endosomal vesicle biogenesis, viral budding and cytokinetic abscission. PDCD6IP is a part of the ESCRT (endosomal sorting complexes required for transport) machinery.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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ALIX is recruited temporarily into HIV-1 budding sites at the end of gag assembly.
Ku PI
PLoS ONE, 9 (2014)
Association between Programmed Cell Death 6 Interacting Protein Insertion/Deletion Polymorphism and the Risk of Breast Cancer in a Sample of Iranian Population.
Hashemi M
Disease Markers (2015)
Jun-Chao Xie et al.
American journal of translational research, 10(1), 150-163 (2018-02-10)
Hypoxia promotes the accumulation of amyloid-β (Aβ), which is related to the pathogenesis of Alzheimer's disease (AD). CD147 is considered as an additional subunit of γ-secretase regulated by hypoxia, and has been identified in exosomes. Aβ is also found in
Lucia Casadei et al.
Journal of extracellular vesicles, 11(9), e12251-e12251 (2022-09-01)
EVs have emerged as an important component in tumour initiation, progression and metastasis. Although notable progresses have been made, the detection of EV cargoes remain significantly challenging for researchers to practically use; faster and more convenient methods are required to
Ádám Oszvald et al.
Stem cells (Dayton, Ohio), 38(2), 291-300 (2019-11-02)
Extracellular vesicles (EV) are membrane-surrounded vesicles that represent a novel way of intercellular communication by carrying biologically important molecules in a concentrated and protected form. The intestinal epithelium is continuously renewed by a small proliferating intestinal stem cell (ISC) population
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