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SAB4200232

Sigma-Aldrich

Anti-GAD65 antibody , Mouse monoclonal

clone N-GAD65, purified from hybridoma cell culture

Synonym(s):

Anti-GAD2, Anti-glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa), Anti-glutamate decarboxylase 65 kDa isoform

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About This Item

MDL number:
MFCD03455043
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

N-GAD65, monoclonal

form

buffered aqueous solution

mol wt

antigen ~65 kDa

species reactivity

human, mouse, monkey, rat

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.25-0.5 μg/mL using whole extracts of rat brain

isotype

IgG1

UniProt accession no.

Q05329

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

Related Categories

General description

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

Application

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

Biochem/physiol Actions

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

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Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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Purification and characterization of GAD65-specific monoclonal autoantibodies [version 1; referees: awaiting peer review]
Jiang W, et al.
F1000Research, 5003(7), 1VL-1VL (2015)
GAD65 autoantibodies and its role as biomarker of Type 1 diabetes and Latent Autoimmune Diabetes in Adults (LADA)
Towns R and Pietropaolo M
Drugs of the Future, 36(11), 847-847 (2011)
Wei Jiang et al.
F1000Research, 4, 135-135 (2015-05-29)
Autoantibodies against antigens expressed by insulin-producing β cells are circulating in both healthy individuals and patients at risk of developing Type 1 diabetes. Recent studies suggest that another set of antibodies (anti-idiotypic antibodies) exists in this antibody/antigen interacting network to regulate
A GABAergic system in airway epithelium is essential for mucus overproduction in asthma
Xiang YY, et al.
Nature Medicine, 13(7), 862-862 (2007)
Association between glutamic acid decarboxylase genes and anxiety disorders, major depression, and neuroticism
Hettema JM, et al.
Molecular Psychiatry, 11(8), 752-752 (2006)
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