SAB4200059
Anti-Chimerin β2 antibody, Rat monoclonal
clone HAR 2E3, purified from hybridoma cell culture
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Synonym(s):
Anti-ARHGAP3, Anti-BCH, Anti-CHN2, Anti-Chimaerin β2, Anti-GTPase-activating protein, RHO, 3, Anti-RHOGAP3, Anti-Rho-GTPase-activating protein 3, Monoclonal Anti-Chimerin β2 antibody produced in rat
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rat
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HAR 2E3, monoclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
human, monkey, rat, mouse, bovine
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
western blot: 1.0-2.0 μg/mL using Neuro-2a cell extracts
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CHN2(1124)
General description
Monoclonal Anti-Chimerin β2 (rat IgG2a isotype) is derived from the hybridoma HAR 2E3 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with a fusion protein of full length human Chimerin β2. Chimerin β2 (CHN2) is a member of the chimerin family and encodes a protein with a phorbol-ester/ diacylglycerol (DAG)-type zinc finger containing C1 domain, a Rho-GTPase activating proteins (GAP) domain and an Src homology 2 (SH2) domain. Chimerin β2 gene is mapped to human chromosome 7p14.3.
Specificity
Monoclonal Anti-Chimerin b2 recognizes human, monkey, bovine, mouse and rat Chimerin b2.
Immunogen
fusion protein of full length human Chimerin β2
Application
Monoclonal Anti-Chimerin β2 antibody produced in rat has been used in immunoblotting at a dilution of 1:1000 and immunocytochemistry.
Biochem/physiol Actions
Chimerin β2 protein (CHN2) is a high affinity receptor for the phorbol esters and diacylglycerol (DAG). Binding of DAG induces translocation of the protein from the cytosol to the Golgi apparatus membrane. It has guanosine triphosphatase GTPase-activating protein activity regulated by phospholipid binding. Decreased expression of CHN2 gene is associated with high-grade gliomas and breast. Deregulation in expression of CHN2 gene is associated with lymphomas, glioblastoma and insulin resistance. Mutations in the CHN2 gene have been associated with schizophrenia in men.
Physical form
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
Storage and Stability
Store at -20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Marcus G Pezzolesi et al.
Kidney international, 80(1), 105-111 (2011-03-18)
A genome-wide association scan of the Genetics of Kidneys in Diabetes (GoKinD) collections identified four novel susceptibility loci, located on chromosomes 7p14.3, 9q21.32, 11p15.4, and 13q33.3 associated with type 1 diabetic nephropathy. A recent evaluation of these loci in Japanese
M J Caloca et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(21), 11854-11859 (1999-10-16)
The members of the chimaerin family of Rac-GTPase-activating proteins possess a single C1 domain with high homology to those present in protein kinase C (PKC) isozymes. This domain in PKCs is involved in phorbol ester and diacylglycerol (DAG) binding. We
A missense polymorphism (H204R) of a Rho GTPase-activating protein, the chimerin 2 gene, is associated with schizophrenia in men.
Ryota Hashimoto et al.
Schizophrenia research, 73(2-3), 383-385 (2005-01-18)
Bertram Canagarajah et al.
Cell, 119(3), 407-418 (2004-10-28)
The lipid second messenger diacylglycerol acts by binding to the C1 domains of target proteins, which translocate to cell membranes and are allosterically activated. Here we report the crystal structure at 3.2 A resolution of one such protein, beta2-chimaerin, a
Victoria Casado-Medrano et al.
Oncotarget, 7(19), 28301-28319 (2016-04-09)
β2-chimaerin is a Rac1-specific negative regulator and a candidate tumor suppressor in breast cancer but its precise function in mammary tumorigenesis in vivo is unknown. Here, we study for the first time the role of β2-chimaerin in breast cancer using
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