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Safety Information

SAB3500043

Sigma-Aldrich

Anti-β-Galactosidase antibody produced in chicken

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-β-gal

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About This Item

MDL number:
MFCD00162155
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

chicken

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

bacteria

technique(s)

indirect ELISA: suitable
western blot: suitable

NCBI accession no.

NP_752394

UniProt accession no.

Q8FKG6

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

β-Galactosidase is coded by the LacZ gene, which helps to transform lactose into galactose. β-Galactosidases are used to produce galactooligosaccharides (GOS). Lactobacillus β-galactosidases are heterodimeric proteins, that are coded by lacL and lacM genes.
β-galactosidase is a tetramer made up of identical four polypeptide chains with a molecular weight of 135,000 each and constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. It cleaves lactose into its monosaccharide components, glucose and galactose. The sulfhydryl containing enzyme also catalyzes the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Immunogen

beta-gal antibody was raised against a 17 amino acid peptide from near the amino terminus of E. coliβ-gal.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Linkage

The action of this antibody can be blocked using blocking peptide SBP3500043.

Physical form

Supplied at approx. 1 mg/mL in phosphate buffered saline containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
51550903

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Structural comparisons of TIM barrel proteins suggest functional and evolutionary relationships between beta-galactosidase and other glycohydrolases
D H Juers
Protein Science (1999)
Characterization of Sulfolobus solfataricus β?galactosidase mutant F441Y expressed in Pichia pastoris.
Sun X, et al.
Journal of the Science of Food and Agriculture, 94(7), 1359-1365 (2014)
PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12
G R CRAVEN
The Journal of Biological Chemistry, 240 (1965)
LacZ ?-galactosidase: structure and function of an enzyme of historical and molecular biological importance
Protein Science (2012)
Production and secretion of Lactobacillus crispatus β-galactosidase in Pichia pastoris.
Nie C, et al.
Protein Expression and Purification, 92(1), 88-93 (2013)
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