Skip to Content
Merck
CN
All Photos(4)

Documents

Safety Information

SAB2702306

Sigma-Aldrich

Monoclonal Anti-Histone H4K20me2 (dimethyl Lys20) antibody produced in mouse

clone GT1851, affinity isolated antibody

Sign Into View Organizational & Contract Pricing
All Photos(4)
Synonym(s):
H4FA
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT1851, monoclonal

form

buffered aqueous solution

mol wt

11kDa

species reactivity

human

concentration

1mg/mL

technique(s)

ChIP: suitable
dot blot: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: 1:1,000-1:10,000

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

dimethylation (Lys20)

Gene Information

human ... HIST1H4A(8359)

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K20me2 (dimethyl Lys20).

Application

Suggested starting dilutions are as follows: ICC/IF: 1:100-1:1000, IP: 1:100-1:500, WB: 1:1000-1:10000, ChIP assay: Assay-dependent dilution. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Biochem/physiol Actions

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

新产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service