SAB2702295
Monoclonal Anti-mCherry antibody produced in mouse
clone GT844, affinity isolated antibody
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UNSPSC Code:
12352203
NACRES:
NA.41
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
GT844, monoclonal
form
buffered aqueous solution
mol wt
27kDa
concentration
1mg/mL
technique(s)
immunofluorescence: 1:100-1:1,000
western blot: 1:500-1:3,000
isotype
IgG2a
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
The acceptor mCherry is a monomeric, red fluorescent protein obtained from the stony-coral Discosoma protein DsRED. It is expressed in the cytoplasm, has a high photostability and is unaffected by photobleaching. mCherry acts as a good FRET (fluorescence resonance energy transfer) acceptor. mCherry allows rapid fluorescence detection after a particular gene is expressed.
Immunogen
Full length mCherry recombinant protein
Application
Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Other Notes
Purification: Affinity purified by Protein G
Physical form
Phosphate-buffered saline, no preservative added.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Quantitative FRET Analysis With the E0GFP?mCherry Fluorescent Protein Pair.
Albertazzi L, et al.
Photochemistry and Photobiology, 85(1), 287-297 (2009)
In vitro screening test using Leishmania promastigotes stably expressing mCherry protein.
Vacchina P and Morales MA
Antimicrobial Agents and Chemotherapy, 58(3), 1825-1828 (2014)
Hang Zhou et al.
Nature neuroscience, 26(3), 458-469 (2023-01-24)
Poor sleep is associated with the risk of developing chronic pain, but how sleep contributes to pain chronicity remains unclear. Here we show that following peripheral nerve injury, cholinergic neurons in the anterior nucleus basalis (aNB) of the basal forebrain
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