S8406
Stromal Cell-Derived Factor 1β/pre-B Cell Growth Stimulating Factor human
>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
SDF-1β/PBSF
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About This Item
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
10-30 ng/mL ED50
mol wt
~8 kDa
packaging
pkg of 10 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CXCL12(6387)
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General description
C-X-C motif chemokine ligand 12 (CXCL12), also known as stromal cell-derived factor 1 α (SDF-1a), is encoded by the gene mapped to human chromosome 10q11. Stromal cell-derived factor 1 α and β (SDF-1 α and β) are stromal derived CXC chemokines and signal through the C-X-C motif chemokine receptor-4 (CXCR4). CXCL12 is mainly expressed in the inflamed and failing myocardium.
Application
Stromal Cell-Derived Factor 1β has been used to activate chemokine receptor-4 (CXCR4) and to further assess its role in tumor cell migration using chemotaxis assay.
Biochem/physiol Actions
C-X-C motif chemokine ligand 12 (CXCL12) is involved in inflammation, proliferation, tumorigenicity and metastasis. CXCL12 exerts HIV (human immunodeficiency virus) suppressive activity in cells expressing the CXCR4 (C-X-C motif chemokine receptor 4). It is overexpressed in breast carcinoma, ovarian carcinoma and papillary thyroid carcinoma. It enhances angiogenesis in the ischemic tissue. SDF1 is also implicated in neuroprotection after ischemic stroke with the help of CXCR4.
SDF-1/PBSF is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. It is highly conserved between species, with only one amino acid substitution between the human and mouse proteins.
SDF-1/PBSF, a member of the α (CXC) chemokine family, is a chemoattractant for T lymphocytes and monocytes, but not neutrophils. SDF-1α and SDF-1β (together also known as SDF-1/PBSF) are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the C-terminal of SDF-1β and absent from SDF-1α. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the human and mouse proteins.
Physical form
Lyophilized from 30% acetonitrile and 0.1% trifluoracetic acid containing 0.5 mg of bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to chemoattract 8-10 day human T lymphocytes cultured in the presence of IL-2.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
Activation of chemokine receptor CXCR4 in malignant glioma cells promotes the production of vascular endothelial growth factor?
Shi-xinYang
Biochemical and Biophysical Research Communications, 523-528 (2005)
Endomyocardial expression of SDF-1 predicts mortality in patients with suspected myocarditis.
Zuern CS
Clinical Research in Cardiology : Official Journal of the German Cardiac Society (2015)
Stromal cell-derived factor 1a (SDF-1a): A marker of disease burden in patients with atrial fibrillation.
Li D
Scandinavian Cardiovascular Journal : SCJ (2016)
Gene-environment interaction effects on the development of immune responses in the 1st year of life.
American Journal of Human Genetics (2005)
HIV coreceptor downregulation as antiviral principle: SDF-1alpha-dependent internalization of the chemokine receptor CXCR4 contributes to inhibition of HIV replication.
Amara A
The Journal of Experimental Medicine (1997)
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