Skip to Content
Merck
CN

S4760

N-Succinyl-Ala-Ala-Ala-p-nitroanilide

elastase substrate, chromogenic, ≥98% (HPLC), powder

Synonym(s):

N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide, N-Succinyl-tri-L-alanine 4-nitroanilide

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

Empirical Formula (Hill Notation):
C19H25N5O8
CAS Number:
52299-14-6
Molecular Weight:
451.43
NACRES:
NA.32
PubChem Substance ID:
24899617
UNSPSC Code:
12352204
EC Number:
257-823-5
MDL number:
MFCD00036774
Beilstein/REAXYS Number:
2928351

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

N-Succinyl-Ala-Ala-Ala-p-nitroanilide, elastase substrate

InChI key

GVUGADOWXGKRAE-SRVKXCTJSA-N

InChI

1S/C19H25N5O8/c1-10(20-15(25)8-9-16(26)27)17(28)21-11(2)18(29)22-12(3)19(30)23-13-4-6-14(7-5-13)24(31)32/h4-7,10-12H,8-9H2,1-3H3,(H,20,25)(H,21,28)(H,22,29)(H,23,30)(H,26,27)/t10-,11-,12-/m0/s1

SMILES string

C[C@H](NC(=O)CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)Nc1ccc(cc1)[N+]([O-])=O

assay

≥98% (HPLC)

form

powder

solubility

DMF: 25 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Application

N-Succinyl-Ala-Ala-Ala-p-nitroanilide has been used as a substrate
  • for screening elastase activity in lyophilized earthworm fibrinolytic enzymes
  • for quantifying elastase activity in sheep milk
  • in screening honeybee inhibitors in serine protease inhibition assay

General description

N-Succinyl-Ala-Ala-Ala-p-nitroanilide is a chromogenic substrate for chymotrypsin-like serine protease, pancreatic elastase and serine endopeptidase.
Elastase substrate.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

常规特殊物品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000521131
0000521133
0000521132
0000521132
0000521133

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Role of endogenous enzymes in proteolysis of sheep milk
Albenzio M, et al.
Journal of Dairy Science, 92(1), 79-86 (2009)
SEP-1-a subtilisin-like serine endopeptidase from germinated seeds of Hordeum vulgare L. cv. Morex
Fontanini D and Jones BL
Planta, 215(6), 885-893 (2002)
Transgenic chickpea expressing a recombinant human a1-proteinase inhibitor (a1-PI) driven by a seed-specific promoters from the common bean Phaseolus vulgaris (L.).
Mishra, S., Jha, S., Singh, R., Chaudhary, S., Sanyal, I., & Amla, D. V.
Plant Cell, Tissue and Organ Culture, 115, 23-33 (2013)
Ulvi Kahraman Gürsoy et al.
Pathogens (Basel, Switzerland), 9(9) (2020-09-19)
Aim was to profile salivary total protease, Porphyromonas gingivalis gingipain, and neutrophil elastase activities in relation to the resolution of periodontal inflammation, salivary macrophage-derived chemokine (MDC), and macrophage inflammatory protein-1α concentrations. Nonsurgical periodontal treatment was performed in 24 periodontitis patients
M Rouis et al.
Arteriosclerosis (Dallas, Tex.), 10(2), 246-255 (1990-03-01)
We investigated the effects of a number of stimulatory agents on the production of both cell-associated and extracellular elastase-type enzymes on human monocyte-macrophages in vitro and of the modulation of such effects by modification of cellular cholesterol content. The stimulatory
View All Related Papers

Articles

Elastase Function and Importance

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Protocols

Enzymatic Assay of Elastase (EC 3.4.21.36)

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service