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Merck
CN

S3504

Sigmacell Cellulose

Type 20, 20 μm

Synonym(s):

Cellulose, Cellulose powder, Cotton linters

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About This Item

CAS Number:
9004-34-6
EC Number:
232-674-9
MDL number:
MFCD00081512
UNSPSC Code:
12352200
NACRES:
NA.56

Key Documents

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type

Type 20

Quality Level

200

particle size

20 μm

SMILES string

O1[C@H](C(C(C(C1CO)O)O)O)O[C@@H]2C(OC(C(C2O)O)O)CO

InChI

1S/C12H22O11/c13-1-3-5(15)6(16)9(19)12(22-3)23-10-4(2-14)21-11(20)8(18)7(10)17/h3-20H,1-2H2/t3?,4?,5?,6?,7?,8?,9?,10-,11?,12+/m1/s1

InChI key

GUBGYTABKSRVRQ-WFVLMXAXSA-N

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Application

High purity cellulose powders for partition chromatography.
Makes extremely durable layers. Sharp separations for amino acids, sugars and other hydrophilic substances. Development time as short as 2 hours for 8′′ plates.
Sigmacell cellulose is used in thin layer chromatography (TLC) and cellulose TLC adsorbents. Sigmacell cellulose has been used in studies to inform industrial applications for large-scale purification of enzymes as well as to investigate the cellulase system of Penicillium echinulatum, which has been identified as a potential cellulase producer for bioconversion processes.

Preparation Note

For TLC, simply blend a 15-20% aqueous slurry for about one minute and coat plates; dry at room temperature. Usually does not require activation before use.

Legal Information

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000502867
0000502867
0000503788
0000503787
0000503788

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Stuart M Linton et al.
The Journal of experimental biology, 207(Pt 23), 4095-4104 (2004-10-23)
Digestive juice from the herbivorous gecarcinid land crabs Gecarcoidea natalis and Discoplax hirtipes exhibited total cellulase activity and activities of two cellulase enzymes; endo-beta-1,4-glucanase and beta-1,4-glucosidase. These enzymes hydrolysed native cellulose to glucose. The digestive juice of both species also
L Brinchi et al.
Carbohydrate polymers, 94(1), 154-169 (2013-04-03)
The use of renewables materials for industrial applications is becoming impellent due to the increasing demand of alternatives to scarce and unrenewable petroleum supplies. In this regard, nanocrystalline cellulose, NCC, derived from cellulose, the most abundant biopolymer, is one of
Yulia Fridman et al.
Genes & development, 28(8), 912-920 (2014-04-17)
Coherent plant growth requires spatial integration of hormonal pathways and cell wall remodeling activities. However, the mechanisms governing sensitivity to hormones and how cell wall structure integrates with hormonal effects are poorly understood. We found that coordination between two types
Eshchar Mizrachi et al.
The New phytologist, 194(1), 54-62 (2012-04-05)
Fast-growing, short-rotation forest trees, such as Populus and Eucalyptus, produce large amounts of cellulose-rich biomass that could be utilized for bioenergy and biopolymer production. Major obstacles need to be overcome before the deployment of these genera as energy crops, including
Rachel A Burton et al.
Current opinion in biotechnology, 26, 79-84 (2014-04-01)
Plant cell walls consist largely of cellulose, non-cellulosic polysaccharides and lignin. Concerted attempts are underway to convert wall polysaccharides from crop plant residues into renewable transport fuels and other valuable products, and to exploit the dietary benefits of cereal grain
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Protocols

Enzymatic Assay of Cellulase

To standardize an enzymatic assay procedure of cellulase.

纤维素酶的酶学测定

该实验方案使用分光光度法测定纤维素酶活性。黑曲霉纤维素酶催化内-1,4-β-D-糖苷键的水解。

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