All Photos(2)
Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.21
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![2-[4-(2-Hydroxyethyl)-1-piperazinyl]-ethanesulfonic acid Buffer substance HEPES EMPROVE® EXPERT](/deepweb/assets/sigmaaldrich/product/images/351/602/c50c8b6f-6bcd-4751-9064-2cc5cb47d00f/640/c50c8b6f-6bcd-4751-9064-2cc5cb47d00f.jpg)
biological source
synthetic
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, microbial, and trace metals; tested
pH
4.7-5.3 (, )
useful pH range
6.8-8.2
pKa (25 °C)
7.5
suitability
suitable for manufacturing use
foreign activity
Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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General description
Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.
M-Clarity Program
Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.
Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
M-Clarity Program
Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.
Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
Application
HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.
HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.
HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.
Packaging
This product is available in the following package sizes:
RES6003H-B701X: 100g container
RES6003H-B703X: 5kg container
RES6003H-B705X: 25kg container
RES6003H-B706X: 50kg container
RES6003H-B701X: 100g container
RES6003H-B703X: 5kg container
RES6003H-B705X: 25kg container
RES6003H-B706X: 50kg container
Legal Information
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany
replaced by
Product No.
Description
Pricing
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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