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54457

Sigma-Aldrich

HEPES

≥99.5% (titration), powder, for molecular biology, BioUltra

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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500 ML
CN¥434.58
1 L
CN¥700.66
6 X 500 ML
CN¥2,085.93
24 X 500 ML
CN¥7,822.27

CN¥434.58

Available to ship onApril 16, 2025Details

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500 ML
CN¥434.58
1 L
CN¥700.66
6 X 500 ML
CN¥2,085.93
24 X 500 ML
CN¥7,822.27

About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
230-907-9
MDL number:
MFCD00006158
UNSPSC Code:
12161700
PubChem Substance ID:
57651473
NACRES:
NA.25

CN¥434.58

Available to ship onApril 16, 2025Details

Request a Bulk Order

Product Name

HEPES, BioUltra, ≥99.5% (T)

grade

Molecular Biology

Quality Level

200

product line

BioUltra

Assay

≥99.5% (T)

form

powder

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue

≤0.1% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H2O)

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Show Differences

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This Item
116807541281644927
3-Chloroperbenzoic acid ≤77%

273031

3-Chloroperbenzoic acid

3-Phenylbutyric acid 98%

116807

3-Phenylbutyric acid

Methaneseleninic acid 95%

541281

Methaneseleninic acid

3-Methylpyrazole-5-carboxylic acid 97%

644927

3-Methylpyrazole-5-carboxylic acid

reaction suitability

reagent type: oxidant

reaction suitability

-

reaction suitability

-

reaction suitability

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

-

mp

69-71 °C (lit.)

mp

35-38 °C (lit.)

mp

128-132 °C (lit.)

mp

236-240 °C (lit.)

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

form

solid

form

solid

form

-

form

solid

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2]

Application

HEPES has been used as a component of:
  • DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells[3]
  • RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)[4]
  • Recapture solution to re-capture RNA exosomes from the glycerol gradient[5]
  • ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation[6]
  • 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors[7]

Other Notes

3′-Terminal labeling of RNA with T4 RNA ligasecitation; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffercitation.
Easily compare specifications for HEPES products with the HEPES specification table.
3′-Terminal labeling of RNA with T4 RNA ligase[8]; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer[9].

comparable product

Product No.
Description
Pricing

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCM9649
    BCCL8226
    BCCK3478
    BCCH8168
    BCCJ2320

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    The correction of the metabolic parameters of msg-induced obesity in rats by 2-[4-(benzyloxy) phenoxy] acetic acid.
    Konopelniuk V, et al.
    Journal of Nutrition and Intermediary Metabolism, 13, 1-9 (2018)
    Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.
    Zhang F, et al.
    Nature Protocols, 5(3), 439-439 (2010)
    Sample Preparation of Telomerase Subunits for Crystallization.
    Huang J, et al.
    Bio-protocol, 5(16) (2015)
    RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells.
    Domanski M and LaCava J
    Bio-protocol, 7(8) (2017)
    Isolation and analysis of nuclear RNA.
    J R Nevins
    Methods in enzymology, 152, 234-241 (1987-01-01)
    View All Related Papers

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