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Sigma-Aldrich

HEPES

≥99.5% (titration), powder, for molecular biology, BioUltra

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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
7365-45-9
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
230-907-9
MDL number:
MFCD00006158
UNSPSC Code:
12161700
PubChem Substance ID:
57651473
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

product line

BioUltra

Assay

≥99.5% (T)

form

powder

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

ign. residue

≤0.1% (as SO4)

loss

≤0.5% loss on drying, 110 °C

pH

5.0-6.5 (25 °C, 1 M in H2O)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

H2O: 1 M at 20 °C, clear, colorless

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.045
λ: 280 nm Amax: 0.035

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used as a component of:
  • DMEM (Dulbecco′s modified Eagle′s medium) used for the cultivation of CHO-GPR40?cells
  • RNA buffer (for gel-filtration chromatography) and transcription buffer (for transcription reaction)
  • Recapture solution to re-capture RNA exosomes from the glycerol gradient
  • ExoI and ExoII extraction solution as well as light and heavy gradient solution for gradient centrifugation
  • 2X HBS buffer that is used in transfection process for the production of recombinant lentivectors

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.
3′-Terminal labeling of RNA with T4 RNA ligase; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer.

comparable product

Product No.
Description
Pricing

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures.
Zhang F, et al.
Nature Protocols, 5(3), 439-439 (2010)
The correction of the metabolic parameters of msg-induced obesity in rats by 2-[4-(benzyloxy) phenoxy] acetic acid.
Konopelniuk V, et al.
Journal of Nutrition and Intermediary Metabolism, 13, 1-9 (2018)
Affinity Purification of the RNA Degradation Complex, the Exosome, from HEK-293 Cells.
Domanski M and LaCava J
Bio-protocol, 7(8) (2017)
Sample Preparation of Telomerase Subunits for Crystallization.
Huang J, et al.
Bio-protocol, 5(16) (2015)
RNA Degradation Assay Using RNA Exosome Complexes, Affinity-purified from HEK-293 Cells.
Domanski M and LaCava J
Bio-protocol, 7(8) (2017)
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