PLED35
GenElute™ Endotoxin-free Plasmid Midiprep Kit
sufficient for 35 purifications
Synonym(s):
GenElute™ Endotoxin-free Plasmid Kits, endo free midiprep plasmid isolation kit, endotoxin free plasmid extraction kit, endotoxin free plasmid isolation kit, endotoxin free plasmid purification kit, midiprep plasmid isolation kit, plasmid extraction kit, plasmid purification kit, Gen Elute
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¥5,541.70
Estimated to ship on2025年4月07日Details
Select a Size
About This Item
¥5,541.70
Estimated to ship on2025年4月07日Details
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usage
sufficient for 35 purifications
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
greener alternative category
storage temp.
15-25°C
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1 of 4
This Item | PLEX15 | NA0410 | NA0600 |
|---|---|---|---|
| usage sufficient for 35 purifications | usage sufficient for 15 purifications | usage sufficient for 25 preparations | usage kit sufficient for 5 preparations |
| greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals |
| sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product | sustainability Greener Alternative Product |
| greener alternative category | greener alternative category , Aligned | greener alternative category | greener alternative category , Aligned |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 15-25°C |
General description
Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 1.2 mg of endotoxin-free plasmid DNA may be prepared from 25-200 mL of culture.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification.
Application
Features and Benefits
- High transfection efficiency and yield
- Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA)
- Fast and simple allowing up to 12 preps in less than 2 hours
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Central nervous system
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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