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P9529

Sigma-Aldrich

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain

lyophilized powder

Synonym(s):

3′,5′-Cyclic-nucleotide 5′-nucleotidohydrolase from bovine brain, PDE

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About This Item

CAS Number:
9040-59-9
Enzyme Commission number:
3.1.4.17 (BRENDA, IUBMB)
EC Number:
232-920-5
MDL number:
MFCD00131864
UNSPSC Code:
12352202

biological source

bovine brain

form

lyophilized powder

specific activity

1-5 units/mg protein (without added calmodulin)

mol wt

~60 kDa

composition

Protein, 4.0-6.0% Lowry

UniProt accession no.

P14100

foreign activity

2′,3′-cyclic nucleotide phosphodiesterase, 5′-nucleotidase and alkaline phosphatase ≤0.1%
5′-ATPase ≤0.2%
inorganic pyrophosphatase ≤0.8%

storage temp.

−20°C

Gene Information

cow ... PDE1A(281969)

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General description

Phosphodiesterase (PDE1) exists in two isoforms -PDE1A2 and PDE1B1. The enzyme has a role in the complex interaction between cyclic nucleotides and Ca2+ second-messenger systems.

Application

Phosphodiesterase, 3′,5′-cyclic-nucleotide-specific from bovine brain has been used to assay the protein activator, calmodulin and in in vitro adenylate cyclase assay.

Biochem/physiol Actions

Hydrolyzes the 3′,5′-phosphodiester in cyclic nucleotide monophosphates, such as cAMP and cGMP, to the corresponding nucleotide 5′-monophosphate.

Packaging

Package size based on activated units

Unit Definition

One unit will hydrolyze 1.0 μmole of 3′,5′-cyclic-AMP to 5′-AMP per min at pH 7.5 at 30 °C.

Physical form

Lyophilized powder containing Tris-HCl buffer salts and lactose

Preparation Note

Affinity purified preparation which has been depleted of calmodulin.

Reconstitution

Reconstitute with 50% glycerol. The total activated units of enzyme will remain constant for at least 5 days when stored at −20 °C. However, the calmodulin-deficient activity may increase up to 200%. Both the activated and calmodulin-deficient activity may decrease approximately 30% in 24 hours if stored at 4 °C.

Analysis Note

Protein determined by Warburg-Christian

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
096K7415
089H7440
071H95205
071H95203
071H95201

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P D Calvert et al.
The Journal of general physiology, 111(1), 39-51 (1998-02-14)
Light adaptation in vertebrate photoreceptors is thought to be mediated through a number of biochemical feedback reactions that reduce the sensitivity of the photoreceptor and accelerate the kinetics of the photoresponse. Ca2+ plays a major role in this process by
M D Houslay
Seminars in cell & developmental biology, 9(2), 161-167 (1998-05-26)
Cyclic AMP has provided the paradigm for the second messenger concept. Recent evidence has identified a complex array of isoforms of the enzymes involved in generation, destroying and transmitting the effects of this second messenger. These proteins provide a sophisticated
Reciprocal regulation of calcium dependent and calcium independent cyclic AMP hydrolysis by protein phosphorylation
Kok-Long Ang Ferenc A. Antoni
Journal of Neurochemistry (2002)
Drummond, G.I. And Yamamoto, M. et al.
The Enzymes (1971)
Vibrio vulnificus Biotype 3 Multifunctional Autoprocessing RTX Toxin Is an Adenylate Cyclase Toxin Essential for Virulence in Mice
Kevin J. Ziolo
Infection (Munich) (2014)
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