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P8982

Sigma-Aldrich

Anti-phospho-p53 (pSer392) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

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MDL number:
MFCD02097164
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

mol wt

antigen 53 kDa

species reactivity

mouse, human

technique(s)

microarray: suitable
western blot (chemiluminescent): 1:1,000 using p53 fusion proteins with CKII phosphorylation or an extract of hydroxyurea-treated MvILu cells

UniProt accession no.

P04637

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TP53(7157)
mouse ... Trp53(22059)

General description

p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Specificity

Does not react with non-phosphorylated p53.

Immunogen

synthetic peptide corresponding to residues around Ser392 of human p53 conjugated to KLH.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

Preparation Note

Purified using protein A and peptide affinity chromatography.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

含少量动物源组分生物产品
常规特殊物品

Certificates of Analysis (COA)

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Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Multiple myeloma involving central nervous system: high frequency of chromosome 17p13.1 (p53) deletions
Hong Chang
British Journal of Haematology, 127(3), 280?284-280?284 (2004)
Mutations in the p53 Tumor Suppressor Gene
Noa Rivlin
Genes & Cancer, 2(4), 466?474-466?474 (2011)
Suppression of p53 potentiates chemosensitivity in nutrient?deprived cholangiocarcinoma cells via inhibition of autophagy
Fei Hui
Oncology, 14.2, 1959-1966 (2017)
View All Related Papers

Articles

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

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