P8913
Phospholipase A2 from bovine pancreas
lyophilized powder, ≥20 units/mg protein
Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-637-7
MDL number:
Product Name
Phospholipase A2 from bovine pancreas, lyophilized powder, ≥20 units/mg protein
form
lyophilized powder
specific activity
≥20 units/mg protein
composition
Protein, 5-20% biuret
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
cow ... PLA2G1B(282457)
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Analysis Note
Protein determined by biuret.
Application
Phospholipase A2 from bovine pancreas has been used to determine rat renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation and to study the impact of phospholipase A2 on tubular cell triglyceride levels.
Biochem/physiol Actions
Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.
General description
Phospholipase A2 from bovine pancreas is a calcium-dependent lipolytic enzyme.
Other Notes
One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.0 and 37 °C.
Packaging
Package size based on protein content
Physical form
Contains Tris buffer salts.
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
低风险生物材料
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Triglyceride accumulation in injured renal tubular cells: alterations in both synthetic and catabolic pathways.
Johnson AC
Kidney International, 67(6), 2196-2209 (2005)
Renal tubular triglyercide accumulation following endotoxic, toxic, and ischemic injury.
Zager RA
Kidney International, 67(1), 111-121 (2005)
Phospholipase A2 activity can protect renal tubules from oxygen deprivation injury.
Zager RA
Proceedings of the National Academy of Sciences of the USA, 90(17), 8297-8301 (1993)
Ming Chen et al.
Scientific reports, 7(1), 15931-15931 (2017-11-23)
Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae. Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The
K Sekar et al.
Acta crystallographica. Section D, Biological crystallography, 62(Pt 4), 392-397 (2006-03-23)
The lipolytic enzyme phospholipase A2 plays a crucial role in lipid metabolism and catalyzes hydrolysis of the fatty-acid ester bond at the sn-2 position of phospholipids. Here, the crystal structure (1.7 A resolution) of the triple mutant (K53,56,121M) of bovine
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