Broad-spectrum antiviral agents: secreted phospholipase A

Broad-spectrum antiviral agents: secreted phospholipase A

Scientific reports (2017-11-23)

Ming Chen, Chie Aoki-Utsubo, Masanori Kameoka, Lin Deng, Yutaka Terada, Wataru Kamitani, Kei Sato, Yoshio Koyanagi, Makoto Hijikata, Keiko Shindo, Takeshi Noda, Michinori Kohara, Hak Hotta

PMID29162867

ABSTRACT

Hepatitis C virus (HCV), dengue virus (DENV) and Japanese encephalitis virus (JEV) belong to the family Flaviviridae. Their viral particles have the envelope composed of viral proteins and a lipid bilayer acquired from budding through the endoplasmic reticulum (ER). The phospholipid content of the ER membrane differs from that of the plasma membrane (PM). The phospholipase A

MATERIALS

Product Number

Brand

Product Description

G5251

Sigma-Aldrich

DL-Glyceraldehyde 3-phosphate solution, 45-55 mg/mL in H₂O

P9279

Sigma-Aldrich

Phospholipase A₂ from honey bee venom (Apis mellifera), salt-free, lyophilized powder, 600-2400 units/mg protein

P8913

Sigma-Aldrich

Phospholipase A₂ from bovine pancreas, lyophilized powder, ≥20 units/mg protein