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P6749

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone PAb1801, purified from hybridoma cell culture

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Synonym(s):
Anti-p53 antibody, Mouse monoclonal, Anti-TRP53, Anti-Transformation-related protein 53
MDL number:
MFCD00164709
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PAb1801, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.03-0.06 μg/mL using total cell extract of A431 cells

isotype

IgG1

UniProt accession no.

P04637

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

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General description

Anti-p53 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma PAb1801 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. Anti-p53 antibody, Mouse monoclonal recognizes human p53.
p53 is a tumor suppressor gene expressed in a wide variety of tissues. It is a tetrameric nuclear DNA-binding phosphoprotein. The gene encoding it is localized on human chromosome 17p13.1 and mouse chromosome 11.

Application

Monoclonal Anti-p53-Biotin antibody produced in mouse has been used for Western Blotting.

Biochem/physiol Actions

Tumor suppressor p53 has the capability to induce cell cycle arrest and has a role in DNA repair, senescence and apoptosis. It binds to Simian vacuolating virus 40 (SV40) T-antigen and human papilloma virus E6 protein. The p53 gene is mutated in various cancers, such as of the breast, ovarian, bladder, colon and lung.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Wen Qiang Chen et al.
American journal of physiology. Heart and circulatory physiology, 293(5), H2836-H2844 (2007-08-21)
Atherosclerotic plaque rupture and thrombosis are the main causes of acute coronary syndrome. In the present study, we investigated whether ultrasound imaging and inflammatory parameters are predictive of plaque rupture in a newly established animal model. We developed a rabbit
Mitochondrial nucleoid clusters protect newly synthesized mtDNA during Doxorubicin- and Ethidium Bromide-induced mitochondrial stress
Lukas Alan
Toxicology and Applied Pharmacology, 302, 31-40 (2016)
Cell Cycle and Apoptosis
Bruna Pucci
Neoplasia, 2(4) (2000)
Yingji Jin et al.
Archives of pharmacal research, 41(4), 438-449 (2018-03-02)
There has been no attempt to apply protein-based markers of exfoliated cervical cells (ECCs) for primary screening of cervical cancer. In the present study, the levels of six tumor-associated protein [TAPs: Sialyl Lewis A (SLeA), Cancer Antigen 15-3 (CA 15-3)
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Articles

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

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